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Inhibition of USP8 (unknown origin) using ubiquitin rhodamine 110 as substrate at 1 to 10 uM by DUBprofiler fluorometric assay
Assay data:2 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of human USP8 using ubiquitin rhodamine 110 as substrate at 10 to 50 uM by fluorometric assay
Assay data:1 Tested
Inhibition of human USP8 (734 to 1110 residues) expressed in Escherichia coli BL21 (DE3) using ubiquitin rhodamine 110 as substrate assessed as remaining enzyme activity at 10 to 50 uM by fluorometric assay
Inhibition of human USP8 using Di-Ub IQF as substrate incubated for 15 mins by K63-2 assay
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of USP8 (unknown origin)
Assay data:3 Active, 3 Activity ≤ 1 µM, 11 Tested
Inhibition of human USP8 using Ub-AMC as substrate
Assay data:13 Active, 8 Activity ≤ 1 µM, 24 Tested
Inhibition of human his tagged USP8 by Westernblot analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of N-terminal His6-tagged USP8 catalytic domain (734 to 1110 residues) in HEK293T intact cells up to 100 uM in presence of HA-Ub-VS probe by HA antibody based Western blotting assay
Inhibition of N-terminal His6-tagged USP8 catalytic domain (734 to 1110 residues) in HEK293T cell lysates up to 100 uM in presence of HA-Ub-VS probe by HA antibody based Western blotting assay
Inhibition of N-terminal His6-tagged USP8 catalytic domain (734 to 1110 residues) in HEK293T intact cells up to 100 uM in presence of HA-Ub-VS probe by USP8 antibody based Western blotting assay
Inhibition of N-terminal His6-tagged USP8 catalytic domain (734 to 1110 residues) in HEK293T cell lysates up to 100 uM in presence of HA-Ub-VS probe by USP8 antibody based Western blotting assay
Binding affinity to N-terminal His6-tagged USP8 catalytic domain (734 to 1110 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) cells using K63-linked di-ubiquitin as substrate by di-ubiquitin cleavage assay
Binding affinity to N-terminal His6-tagged USP8 catalytic domain (734 to 1110 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) cells by surface plasmon resonance method
Assay data:1 Active, 2 Tested
Inhibition of N-terminal His6-tagged USP8 (unknown origin) catalytic domain (734 to 1110 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as deubiquitinating activity using ubiquitin-rhodamine -110 as substrate incubated for 30 mins followed by substrate addition measured after 30 mins by ubiquitin-rhodamine-110 based fluorometric assay
Assay data:5 Active, 20 Tested
Inhibition of N-terminal His6-tagged USP8 (unknown origin) expressed in Escherichia coli BL21 (DE3) cells using ubiquitin-rhodamine -110 as substrate incubated for 15 mins followed by substrate addition measured after 1 hrs by ubiquitin-rhodamine-110 based fluorometric assay
Inhibition of human full length USP8 overexpressed in baculovirus-infected insect cells using ubiquitin-C-terminal 7-amido-4-methylcoumarin as substrate by fluorescence spectroscopy
Assay data:4 Active, 4 Activity ≤ 1 µM, 4 Tested
Inhibition of USP8 in human NCI-H1975 cells assessed as reduction in EGFR protein level at 500 nM measured for 24 hrs by immunoblotting analysis
Assay data:2 Active, 2 Tested
Inhibition of USP8 in human NCI-H1975 cells assessed as reduction in protein level at 500 nM measured for 24 hrs by immunoblotting analysis
Inhibition of N-terminal His-6 tagged USP8 (734 to 1110 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) cells measured after 3 hrs by fluorescence based Plate reader method
Assay data:4 Active, 1 Activity ≤ 1 nM, 4 Activity ≤ 1 µM, 4 Tested
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