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Inhibition of UCHL1 (unknown origin) using ubiquitin rhodamine 110 as substrate at 1 to 10 uM by DUBprofiler fluorometric assay
Assay data:2 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of human his tagged UCH-L1 by Westernblot analysis
Assay data:1 Tested
Inhibition of UCH-L1 (unknown origin) at 2.5 uM
Inhibition of UCH-L1 (unknown origin) at 10 uM using ubiquitin-rhodamine 110 as substrate preincubated for 30 mins followed by substrate addition and measured after 2 hrs by microplate spectrophotometer analysis
Assay data:6 Tested
Inhibition of UCHL1 (unknown origin) using Ub-Lys-TAMRA as substrate preincubated for 30 mins followed by substrate addition by fluorescence polarization assay
Inhibition of UCHL1 (unknown origin) using Ub-Rho as substrate preincubated for 30 mins followed by substrate addition
Assay data:2 Active, 1 Activity ≤ 1 µM, 2 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of UCHL1 (unknown origin) using Ub-Rho-morpholine as substrate preincubated for 30 mins followed by substrate addition by fluorescence based assay
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
Inhibition of UCHL1 (unknown origin)
Binding affinity to UCHL1 in human HEK293 cells at 130 to 500 nM in presence of ABP MT16-205 by LFQ-NanoLC-MS/MS analysis
Inhibition of UCHL1 in human HEK293 cells at 130 to 2000 nM in presence of ABP MT16-205 by Competitive pulldown based immunoblotting analysis
Binding affinity to UCHL1 in human HEK293 cells assessed as fold change at 30 nM incubated for 3 to 18 hrs by LFQ-chemical proteomics analysis
Binding affinity to UCHL1 in human HEK293 cells assessed as fold change at 130 nM incubated for 3 to 6 hrs by LFQ-chemical proteomics analysis
Binding affinity to UCHL1 in human HEK293 cells at 250 nM incubated for 6 hrs in presence of BME by TAMRA labelled In-gel fluorescence analysis
Binding affinity to UCHL1 in human HEK293 cells at 31 nM by TAMRA labelled pulldown based immunoblotting analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Binding affinity to UCHL1 in human HEK293 cells at 31 nM by TAMRA labelled pulldown based In-gel fluorescence analysis
Binding affinity to UCHL1 in human HEK293 cells at 40 nM incubated for 3 hrs by In-gel fluorescence analysis
Inhibition of UCHL1 in human HEK293 cells using HA-Ub-VME as substrate at 0.1 to 10 uM incubated for 1 hr by immunoblotting analysis
Assay data:2 Active, 2 Tested
Inhibition of UCHL1 in human HEK293 cells assessed as reduction in UCHL1 expression level using HA-Ub-VME as substrate at 0.001 to 30 uM incubated for 1 hr by immunoblotting analysis
Inhibition of UCHL1 in human HEK293 cells assessed as reduction in UCHL1-Ub level using HA-Ub-VME as substrate at 0.001 to 30 uM incubated for 1 hr by immunoblotting analysis
Inhibition of FLAG-tagged UCHL1 (unknown origin) expressed in CAL-51 cells using HA-Ub-VME as substrate incubated for 30 mins by HTRF assay
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
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