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Inhibition of USP28 (unknown origin) using ubiquitin rhodamine 110 as substrate at 1 to 10 uM by DUBprofiler fluorometric assay
Assay data:2 Tested
SummaryRelated BioAssays by Target
Inhibition of USP28 (unknown origin) using Ub-Rho100 as substrate by calorimetric analysis
Assay data:3 Active, 1 Activity ≤ 1 µM, 4 Tested
Ubiquitin-Rhodamine110 Assay from US Patent US20230365583: "FUSED TRICYCLIC PYRIMIDINE-THIENO-PYRIDINE SMALL MOLECULE INHIBITORS OF UBIQUITIN-SPECIFIC PROTEASE 28"
Assay data:43 Active, 37 Activity ≤ 1 µM, 49 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
Inhibition of USP28 (unknown origin) at 10 uM using ubiquitin-rhodamine 110 as substrate preincubated for 30 mins followed by substrate addition and measured after 2 hrs by microplate spectrophotometer analysis
Assay data:6 Tested
SummaryPubMed CitationRelated BioAssays by Target
Biochemical Assay from US Patent US11325917: "Thienopyrazine carboxamides as ubiquitin-specific protease inhibitors"
Assay data:22 Active, 7 Activity ≤ 1 µM, 22 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by DepositorRelated BioAssays by Target
Biochemical Assay from US Patent US10913753: "Thienopyridine carboxamides as ubiquitin-specific protease inhibitors"
Assay data:216 Active, 77 Activity ≤ 1 µM, 219 Tested
Biochemical Assay from US Patent US10889592: "Thienopyrazine carboxamides as ubiquitin-specific protease inhibitors"
Assay data:21 Active, 7 Activity ≤ 1 µM, 21 Tested
Inhibition of USP28 (unknown origin) at 20 uM
Inhibition of USP28 (unknown origin) at 10 uM relative to control
Inhibition of human GST-tagged USP28 expressed in Escherichia coli assessed as cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ubiquitin-Rhodamine110-glycine as substrate by fluorescence based analysis
Assay data:1 Tested
USP28 deubiquitinase inhibition: Dose-response Confirmation
Assay data:1416 Active, 1869 Tested
SummaryCompounds, ActiveRelated BioAssays by DepositorRelated BioAssays by Same ProjectRelated BioAssays by Target
USP28 deubiquitinase inhibition: Primary qHTS
Assay data:1413 Active, 47480 Tested
Inhibition of TEV-cleavable hexa-histidine tagged USP28 (1 to 1077 residues) (unknown origin) using ubiquitin-rhodamine 110 as substrate and measured after 90 mins by fluorescence based assay
Assay data:223 Active, 185 Activity ≤ 1 µM, 223 Tested
Inhibition of TEV-cleavable hexa-histidine tagged USP28 (1 to 1077 residues) (unknown origin) using ubiquitin-rhodamine 110 as substrate preincubated for 30 mins followed by substrate addition and measured after 20 mins by fluorescence based assay
Assay data:21 Active, 15 Activity ≤ 1 µM, 21 Tested
Inhibition of USP28 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate measured after 2 to 30 mins by Western blot analysis
Inhibition of USP28 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate preincubated for 19 to 60 mins followed by substrate addition and measured after 19 to 25 mins by Western blot analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Covalent binding affinity to USP28 in human U2OS cells at 20 uM using probe-1 by ABPP analysis
Inhibition of USP28 in human HeLa cell extracts assessed as inhibition of HA-Ub-C2Br probe labeling at 1 to 100 uM incubated for 30 mins by immunoblot analysis
Assay data:2 Active, 2 Tested
Inhibition of USP28 (unknown origin)
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of USP28 by Ub-CHOP reporter assay
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