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Inhibition of human recombinant N-terminal His6-tagged eIF4A R110K mutant expressed in Escherichia coli BL21 CodonPlus cells using ATP and yeast RNA as substrate preincubated with enzyme for 20 mins followed by susbtrate addition and measured after 4 hrs by Malachite green ATPase assay
Assay data:1 Active, 1 Tested
SummaryRelated BioAssays by Target
Inhibition of helicase activity of human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells using Cy3-labeled RNA strand and unlabeled DNA strand by native PAGE analysis
Assay data:1 Active, 2 Tested
Inhibition of helicase activity of human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells assessed as decrease in single stranded signal using Cy3-labeled RNA strand and unlabeled DNA strand by native PAGE analysis
Assay data:2 Active, 2 Tested
Inhibition of helicase activity of human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells assessed as increase in double stranded signal using Cy3-labeled RNA strand and unlabeled DNA strand by native PAGE analysis
Binding affinity to human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells assessed as increase in melting temperature at 3.13 to 50 uM incubated for 5 mins by differential scanning fluorimetry
Displacement of FAM-[AG]8 from human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells preincubated with enzyme for 20 mins followed by FAM-[AG]8 addition for 30 mins by fluorescence polarization assay
Displacement of FAM-A-[CAA]5 from human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells preincubated with enzyme for 20 mins followed by FAM-A[CAA]5 addition for 30 mins by fluorescence polarization assay
Assay data:2 Tested
Displacement of MANT-ATP from human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells at 1 mM preincubated with enzyme and yeast RNA for 20 mins followed by MANT-ATP addition for 30 mins by fluorescence polarization assay
Displacement of MANT-ATP from human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells at 200 uM preincubated with enzyme and yeast RNA for 20 mins followed by MANT-ATP addition for 30 mins by fluorescence polarization assay
Assay data:1 Tested
Binding affinity to eIF4A in human A549 cells assessed as increase in thermal stability of the protein incubated for 90 mins by CETSA analysis
Binding affinity to eIF4A in human A549 cells assessed as increase in thermal stability of the protein at 100 nM incubated for 90 mins by CETSA analysis
Inhibition of human recombinant N-terminal His6-tagged eIF4A R311E mutant expressed in Escherichia coli BL21 CodonPlus cells using ATP and yeast RNA as substrate preincubated with enzyme for 20 mins followed by susbtrate addition and measured after 4 hrs by Malachite green ATPase assay
Inhibition of human recombinant N-terminal His6-tagged eIF4A R311K mutant expressed in Escherichia coli BL21 CodonPlus cells using ATP and yeast RNA as substrate preincubated with enzyme for 20 mins followed by susbtrate addition and measured after 4 hrs by Malachite green ATPase assay
Inhibition of human recombinant N-terminal His6-tagged eIF4A T158V mutant expressed in Escherichia coli BL21 CodonPlus cells using ATP and yeast RNA as substrate preincubated with enzyme for 20 mins followed by susbtrate addition and measured after 4 hrs by Malachite green ATPase assay
Inhibition of human recombinant N-terminal His6-tagged eIF4A T158A mutant expressed in Escherichia coli BL21 CodonPlus cells using ATP and yeast RNA as substrate preincubated with enzyme for 20 mins followed by susbtrate addition and measured after 4 hrs by Malachite green ATPase assay
Inhibition of human recombinant N-terminal His6-tagged eIF4A R110E mutant expressed in Escherichia coli BL21 CodonPlus cells using ATP and yeast RNA as substrate preincubated with enzyme for 20 mins followed by susbtrate addition and measured after 4 hrs by Malachite green ATPase assay
Inhibition of human recombinant N-terminal His6-tagged eIF4A R110M mutant expressed in Escherichia coli BL21 CodonPlus cells using ATP and yeast RNA as substrate preincubated with enzyme for 20 mins followed by susbtrate addition and measured after 4 hrs by Malachite green ATPase assay
Inhibition of human recombinant N-terminal His6-tagged eIF4A R110L mutant expressed in Escherichia coli BL21 CodonPlus cells using ATP and yeast RNA as substrate preincubated with enzyme for 20 mins followed by susbtrate addition and measured after 4 hrs by Malachite green ATPase assay
Competitive inhibition of human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells assessed as decrease in Km with constant Vmax of RNA using 250 uM ATP and varying concentration of yeast RNA as substrate preincubated with enzyme for 20 mins followed by substrate addition and measured after 1 to 7 hrs by Malachite green ATPase assay
Competitive inhibition of human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells using 250 uM ATP and varying concentration of yeast RNA as substrate preincubated with enzyme for 20 mins followed by substrate addition and measured after 1 to 7 hrs by Malachite green ATPase assay
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