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Inhibition of HSET in human MDA-MB-231 cells overexpressing HSET and possessing supernumerary centrosomes assessed as inhibition of centrosome clustering by measuring increase in multipolar spindle formation incubated for 24 hrs by Hoechst 33342 staining based fluorescence microscopic method
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of HSET in human MDA-MB-231 cells overexpressing HSET and possessing supernumerary centrosomes assessed as inhibition of centrosome clustering by measuring increase in multipolar spindle formation at 250 uM incubated for 24 hrs by Hoechst 33342 staining based fluorescence microscopic method
Assay data:2 Tested
Inhibition of HSET in human MDA-MB-231 cells overexpressing HSET and possessing supernumerary centrosomes assessed as inhibition of centrosome clustering by measuring increase in multipolar spindle formation at 40 uM incubated for 24 hrs by Hoechst 33342 staining based fluorescence microscopic method relative to control
Inhibition of HSET in human MDA-MB-231 cells overexpressing HSET and possessing supernumerary centrosomes assessed as inhibition of centrosome clustering by measuring increase in multipolar spindle formation at 20 uM incubated for 24 hrs by Hoechst 33342 staining based fluorescence microscopic method relative to control
Inhibition of HSET in human MDA-MB-231 cells overexpressing HSET and possessing supernumerary centrosomes assessed as inhibition of centrosome clustering by measuring bipolar spindle formation at 40 uM incubated for 24 hrs by Hoechst 33342 staining based fluorescence microscopic method relative to control
Inhibition of HSET in human MDA-MB-231 cells overexpressing HSET and possessing supernumerary centrosomes assessed as inhibition of centrosome clustering by measuring bipolar spindle formation at 20 uM incubated for 24 hrs by Hoechst 33342 staining based fluorescence microscopic method relative to control
Inhibition of recombinant His6-tagged HSET (unknown origin) assessed as reduction in Alexa-594 labelled microtubule gliding at 30 uM measured every 2 secs for 20 secs by epifluorescence microscopic analysis relative to control
Inhibition of KIFC1 in human BT549 cells assessed as induction of multipolar spindle formation at 0.4 uM after 44 hrs
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of KIFC1 in human HeLa cells assessed as induction of multipolar spindle formation at 0.4 uM after 44 hrs
Inhibition of KIFC1 in human HeLa cells assessed as reversal of monospindle phenotype
Inhibition of recombinant N-terminal His6-tagged KIFC1 (Q305-K673 aa) (unknown origin) expressed in Escherichia coli BL21 (DE3) by malachite green assay
Assay data:22 Active, 11 Activity ≤ 1 µM, 37 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of HSET at 10 uM by puruvate kinsase-lactate dehydrogenase detection system
Assay data:3 Tested
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