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GO Biochemical Assay from US Patent US20240091200: "Heterocyclic Carboxylate Compounds as Glycolate Oxidase Inhibitors"
Assay data:12 Active, 175 Tested
SummaryCompounds, ActiveRelated BioAssays by Target
In-Vitro Human Glycolate Oxidase (hGOX) Assay from US Patent US11504367: "Compounds and methods for treating oxalate-related diseases"
Assay data:144 Active, 20 Activity ≤ 1 nM, 158 Activity ≤ 1 µM, 159 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
Non-competitive mixed inhibition of N-terminal His-tagged mouse recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells assessed as decrease in Vmax with increase in apparent Km using glycolate as substrate upto 60 microM
Assay data:4 Active, 4 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Non-competitive mixed inhibition of N-terminal His-tagged human recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells assessed as decrease in Vmax with increase in apparent Km using glycolate as substrate upto 60 microM
Reversible inhibition of N-terminal His-tagged human recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells assessed as enzyme recovery using glycolate as substrate at 100 times of IC50 concentration preincubated for 30 mins followed by substrate addition and measured every 60 sec for 15 mins by jump-dilution based Amplex red and horseradish peroxidase fluorescence assay
Assay data:3 Active, 3 Tested
Reversible inhibition of N-terminal His-tagged human recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells assessed as enzyme recovery using glycolate as substrate at 10 times of IC50 concentration preincubated for 30 mins followed by substrate addition and measured every 60 sec for 15 mins by jump-dilution based Amplex red and horseradish peroxidase fluorescence assay
Non-competitive mixed inhibition of N-terminal His-tagged mouse recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells assessed as inhibition constant using varying concentration of glycolate as substrate measured every 60 sec for 15 mins by Amplex red and horseradish peroxidase based fluorescence assay
Assay data:1 Active, 4 Tested
Non-competitive mixed inhibition of N-terminal His-tagged human recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells assessed as inhibition constant using varying concentration of glycolate as substrate measured every 60 sec for 15 mins by Amplex red and horseradish peroxidase based fluorescence assay
Assay data:2 Active, 4 Tested
Inhibition of N-terminal His-tagged human recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells using glycolate as substrate at 20 uM preincubated for 10 mins followed by substrate addition and measured after 10 mins by Amplex red and horseradish peroxidase based fluorescence assay relative to control
Assay data:2 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of N-terminal His-tagged mouse recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells using glycolate as substrate at 20 uM preincubated for 10 mins followed by substrate addition and measured after 10 mins by Amplex red and horseradish peroxidase based fluorescence assay relative to control
Inhibition of N-terminal His-tagged mouse recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells using glycolate as substrate preincubated for 10 mins followed by substrate addition and measured after 10 mins by Amplex red and horseradish peroxidase based fluorescence assay
Assay data:7 Active, 1 Activity ≤ 1 µM, 8 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of N-terminal His-tagged human recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells using glycolate as substrate preincubated for 10 mins followed by substrate addition and measured after 10 mins by Amplex red and horseradish peroxidase based fluorescence assay
Assay data:4 Active, 8 Tested
Inhibition of N-terminal His-tagged mouse recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells using glycolate as substrate at 20 uM preincubated for 5 mins followed by substrate addition and measured after 1 min in the presence of DCHBS by horseradish peroxidase coupled colorimetric method relative to control
Assay data:6 Tested
Inhibition of N-terminal His-tagged human recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells using glycolate as substrate at 20 uM preincubated for 5 mins followed by substrate addition and measured after 1 min in the presence of DCHBS by horseradish peroxidase coupled colorimetric method relative to control
Inhibition of human recombinant HAO1 expressed in CHO cells co-transformed with a vector expressing glycolate oxidase assessed as cell rescue from CHO-Go induced oxalate mediated cell death incubated for 1 hr followed by addition of glycolic acid and measured upto 96 hrs
Assay data:1 Tested
Inhibition of human recombinant HAO1 expressed in CHO cells co-transformed with a vector expressing glycolate oxidase assessed as cell rescue from CHO-Go induced oxalate mediated cell death at 30 uM incubated for 1 hr followed by addition of glycolic acid and measured upto 96 hrs
Inhibition of human recombinant HAO1 assessed as fluorescent intensity using hydrogen peroxide as substrate by HRP interference detection assay
Assay data:4 Tested
Inhibition of human recombinant HAO1 assessed as detecting hydrogen peroxide using glycolic acid as substrate preincubated for 30 min followed by substrate addition measured after 15 mins by microplate reader
Assay data:24 Active, 19 Activity ≤ 1 µM, 33 Tested
HepaRG-CAR Cell-Based Assay for Quantitation of Glycolate Oxidase Inhibition from US Patent US11389456: "Compounds and methods for treating oxalate-related diseases"
Assay data:16 Active, 3 Activity ≤ 1 µM, 25 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by DepositorRelated BioAssays by Target
In-Vitro Human Glycolate Oxidase (hGOX) Assay from US Patent US11389456: "Compounds and methods for treating oxalate-related diseases"
Assay data:96 Active, 91 Activity ≤ 1 µM, 104 Tested
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