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Inhibition of recombinant Mycobacterium tuberculosis DHFR using dihydrofolate as substrate preincubated for 1 min followed by substrate addition and further incubated for 2 mins in presence of NADPH by spectrophotometer
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryRelated BioAssays by Target
Inhibition of Mycobacterium tuberculosis DHFR
Assay data:4 Active, 1 Activity ≤ 1 nM, 4 Activity ≤ 1 µM, 8 Tested
Potency index, ratio of trimethoprim IC50 to test compound IC50 for recombinant Mycobacterium tuberculosis DHFR expressed in Escherichia coli BL21(DE3) cells using DHF as substrate and NADPH as cofactor by fluorimetric analysis
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Potency index, ratio of MB872 IC50 to test compound IC50 for recombinant Mycobacterium tuberculosis DHFR expressed in Escherichia coli BL21(DE3) cells using DHF as substrate and NADPH as cofactor by fluorimetric analysis
Assay data:6 Tested
Uncompetitive inhibition of Mycobacterium tuberculosis DHFR assessed as varying Vmax using DHF as substrate and NADPH as cofactor by Michaelis-Menten analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Uncompetitive inhibition of Mycobacterium tuberculosis DHFR assessed as constant Km using DHF as substrate and NADPH as cofactor by Michaelis-Menten analysis
Inhibition of recombinant Mycobacterium tuberculosis DHFR expressed in Escherichia coli BL21(DE3) cells using DHF as substrate and NADPH as cofactor by fluorimetric analysis
Assay data:1 Active, 22 Tested
Inhibition of Mycobacterium tuberculosis H37Rv DHFR expressed in Escherichia coli BL21(DE3) assessed as reduction in consumption of NADPH using DHF as substrate preincubated for 1 min followed by DHF addition and measured for 2 mins by spectrophotometric method
Assay data:2 Active, 1 Activity ≤ 1 µM, 4 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of Mycobacterium tuberculosis H37Rv DHFR
Inhibition of Mycobacterium tuberculosis His-tagged DHFR assessed as reduction in consumption of NADPH using DHF as substrate measured every 30 secs over 6 mins by spectrophotometric method
Assay data:4 Active, 2 Activity ≤ 1 µM, 5 Tested
Inhibition of Mycobacterium tuberculosis H37Rv DHFR assessed as reduction in consumption of NADPH using DHF as substrate incubated for 3 mins measured for 2 mins by spectrophotometric method
Assay data:2 Active, 2 Tested
Inhibition of Mycobacterium tuberculosis H37Rv DHFR expressed in Escherichia coli assessed as reduction in consumption of NADPH using DHF as substrate by fluorescence method
Assay data:3 Active, 3 Activity ≤ 1 µM, 3 Tested
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
Inhibition of recombinant Mycobacterium tuberculosis DHFR using 45 uM DHF as substrate by spectrophotometry
Assay data:1 Active, 1 Activity ≤ 1 µM, 2 Tested
Inhibition of Mycobacterium tuberculosis dihydrofolate reductase
Assay data:1 Active, 1 Activity ≤ 1 nM, 1 Activity ≤ 1 µM, 1 Tested
Inhibition of Mycobacterium avium DHFR at IC50 by continuous spectrophotometric assay
Assay data:9 Tested
Inhibition of Mycobacterium avium DHFR by continuous spectrophotometric assay
Assay data:7 Active, 5 Activity ≤ 1 µM, 17 Tested
Inhibition of Mycobacterium avium DHFR at 196 uM under 37 degC by spectrophotometry
Inhibition of Mycobacterium avium DHFR at 37 degC by spectrophotometry
Assay data:3 Active, 3 Activity ≤ 1 µM, 12 Tested
Inhibition of Mycobacterium avium DHFR at 31 uM under 37 degC by spectrophotometry
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