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Inhibition of eIF4E-mediated translation in luciferase RNA primed rabbit reticulocyte lysate assessed as reduction in luciferase production incubated for 90 mins by agarose gel electrophoresis
Assay data:1 Active, 23 Tested
SummaryCompounds, ActivePubMed Citation
Inhibition of P32-[alpha-GTP] cap radiolabelled RNA binding to recombinant eIF4E (unknown origin) assessed as decrease in cross linked RNA-eIF4E complex formation preincubated with UV radiation for 30 mins followed by RNase addition and measured after 30 mins by SDS-PAGE based autoradiographic analysis
Assay data:2 Active, 1 Activity ≤ 1 µM, 23 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of m7GDP fluorescent probe binding to recombinant eIF4E (unknown origin) assessed as dissociation constant by fluorescence polarization assay
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