Warning: The NCBI web site requires JavaScript to function. more...
An official website of the United States government
The .gov means it's official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.
The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.
Inhibition of Thermus thermophilus HB8 GCYH1A expressed in Escherichia coli BL21 (DE3) using GTP as substrate incubated for 60 to 180 mins by spectrofluorimetric analysis
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed Citation
Inhibition of N-terminal His6-tagged Thermus thermophilus HB8 MqnE expressed in Escherichia coli BL21 (DE3) co-expressing [4Fe-4S]2+ cluster using 3-(1-carboxyvinyloxy)benzoic acid as substrate preincubated for 15 mins in presence of SAM and substrate followed by Ti(3+)citrate addition and measured by discontinuous HPLC analysis
Assay data:1 Active, 2 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Reversible inhibition of N-terminal His6-tagged Thermus thermophilus HB8 MqnE expressed in Escherichia coli BL21 (DE3) co-expressing [4Fe-4S]2+ cluster assessed as restoration of enzyme activity at 2 mM using 3-(1-carboxyvinyloxy)benzoic acid as substrate preincubated for 1 hr in presence of SAM, substrate and Ti(3+)citrate followed by compound washout and subsequent addition of SAM, substrate and Ti(3+)citrate measured after 1 hr by HPLC analysis
Assay data:1 Active, 1 Tested
Substrate activity at N-terminal His6-tagged Thermus thermophilus HB8 MqnE expressed in Escherichia coli BL21 (DE3) co-expressing [4Fe-4S]2+ cluster assessed as enzyme-mediated 3-(4-((2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl)-2-carboxybutyl)benzoic acid formation
Inhibition of recombinant Thermus thermophilus HB8 IleRS H581N/L583F double mutant assessed as suppression of L-[14C]isoleucyl-tRNA(Ile) formation
SummaryCompounds, ActiveRelated BioAssays by Target
Inhibition of recombinant Thermus thermophilus HB8 IleRS H581S/L583I double mutant assessed as suppression of L-[14C]isoleucyl-tRNA(Ile) formation
Assay data:1 Tested
SummaryRelated BioAssays by Target
Inhibition of recombinant Thermus thermophilus HB8 IleRS H581L/L583H double mutant assessed as suppression of L-[14C]isoleucyl-tRNA(Ile) formation
Inhibition of recombinant Thermus thermophilus HB8 IleRS L583F mutant assessed as suppression of L-[14C]isoleucyl-tRNA(Ile) formation
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
Inhibition of recombinant Thermus thermophilus HB8 wild type IleRS assessed as suppression of L-[14C]isoleucyl-tRNA(Ile) formation
Activity of Thermus thermophilus HB8 MetRS by ATP:PPi exchange assay in presence of ATP
SummaryPubMed CitationRelated BioAssays by Target
Activity of Thermus thermophilus HB8 MetRS by ATP:PPi exchange assay in presence of methionine
Inhibition of Thermus thermophilus HB8 IPMDH assessed as formation of NADH by Lineweaver-Burke plot analysis
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Filters: Manage Filters
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on