This family consists of two main Cytidylyltransferase activities: 1) 3-deoxy-manno-octulosonate cytidylyltransferase, [3], EC:2.7.7.38 catalysing the reaction:- CTP + 3-deoxy-D-manno-octulosonate <=> diphosphate + CMP-3-deoxy-D-manno-octulosonate, 2) acylneuraminate cytidylyltransferase EC:2.7.7.43, [1,2], catalysing the reaction:- CTP + N-acylneuraminate <=> diphosphate + CMP-N-acylneuraminate. NeuAc cytydilyltransferase of Mannheimia haemolytica has been characterised describing kinetics and regulation by substrate charge, energetic charge and amino-sugar demand[4]. [1]. 9689047. Mammalian cytidine 5'-monophosphate N-acetylneuraminic acid. synthetase: a nuclear protein with evolutionarily conserved. structural motifs.. Munster AK, Eckhardt M, Potvin B, Muhlenhoff M, Stanley P,. Gerardy-Schahn R;. Proc Natl Acad Sci U S A 1998;95:9140-9145.. [2]. 8663048. Purification, cloning, and expression of a cytidine. 5'-monophosphate N-acetylneuraminic acid synthetase from. Haemophilus ducreyi.. Tullius MV, Munson RS Jr, Wang J, Gibson BW;. J Biol Chem 1996;271:15373-15380.. [3]. 7543480. Expression of genes kdsA and kdsB involved in. 3-deoxy-D-manno-octulosonic acid metabolism and biosynthesis of. enterobacterial lipopolysaccharide is growth phase regulated. primarily at the transcriptional level in Escherichia coli K-12.. Strohmaier H, Remler P, Renner W, Hogenauer G;. J Bacteriol 1995;177:4488-4500.. [4]. 11577688. Kinetic properties of the acylneuraminate cytidylyltransferase. from Pasteurella haemolytica A2.. Bravo IG, Barrallo S, Ferrero MA, Rodriguez-Aparicio LB,. Martinez-Blanco H, Reglero A;. Biochem J 2001;358:585-598. (from Pfam)
- Date:
- 2024-08-14