Protein Family Models

Date:

2023-12-12

Date:

2023-12-12

The small mechanosensitive channel, MscS, is a part of the turgor-driven solute efflux system that protects bacteria from lysis in the event of osmotic shock. The MscS protein alone is sufficient to form a functional mechanosensitive channel gated directly by tension in the lipid bilayer. The MscS proteins are heptamers of three transmembrane subunits with seven converging M3 domains, and this domain is one of the inner membrane domains. [1]. 10202137. Protection of Escherichia coli cells against extreme turgor by. activation of MscS and MscL mechanosensitive channels:. identification of genes required for MscS activity.. Levina N, Totemeyer S, Stokes NR, Louis P, Jones MA, Booth IR;. EMBO J. 1999;18:1730-1737.. [2]. 12080120. Purification of the small mechanosensitive channel of. Escherichia coli (MscS): the subunit structure, conduction, and. gating characteristics in liposomes.. Sukharev S;. Biophys J. 2002;83:290-298.. [3]. 16079835. A possible unifying principle for mechanosensation.. Kung C;. Nature. 2005;436:647-654. (from Pfam)

Date:

2024-04-03

The small mechanosensitive channel, MscS, is a part of the turgor-driven solute efflux system that protects bacteria from lysis in the event of osmotic shock. The MscS protein alone is sufficient to form a functional mechanosensitive channel gated directly by tension in the lipid bilayer. The MscS proteins are heptamers of three transmembrane subunits with seven converging M3 domains, and this MscS_porin is towards the N-terminal of the molecules. The high concentration of negative charges at the extracellular entrance of the pore helps select the cations for efflux. [1]. 10202137. Protection of Escherichia coli cells against extreme turgor by. activation of MscS and MscL mechanosensitive channels:. identification of genes required for MscS activity.. Levina N, Totemeyer S, Stokes NR, Louis P, Jones MA, Booth IR;. EMBO J. 1999;18:1730-1737.. [2]. 12080120. Purification of the small mechanosensitive channel of. Escherichia coli (MscS): the subunit structure, conduction, and. gating characteristics in liposomes.. Sukharev S;. Biophys J. 2002;83:290-298.. [3]. 16079835. A possible unifying principle for mechanosensation.. Kung C;. Nature. 2005;436:647-654. (from Pfam)

Date:

2024-04-03

This entry includes small conductance mechanosensitive channels (MscS), which assembles to a homoheptameric complex [1-6]. Each monomer consists of a N-terminal transmembrane region and a large cytoplasmic segment composed of a five-stranded antiparallel beta-sheet (middle/second) domain and a C-terminal alpha/beta-domain [4-7]. This is the beta domain which adopts a SH3-like barrel structure. [1]. 11296222. Structural and functional differences between two homologous. mechanosensitive channels of Methanococcus jannaschii.. Kloda A, Martinac B;. EMBO J 2001;20:1888-1896.. [2]. 11159397. Molecular identification of a mechanosensitive channel in. archaea.. Kloda A, Martinac B;. Biophys J 2001;80:229-240.. [3]. 11275684. Mechanosensitive channels in prokaryotes.. Martinac B;. Cell Physiol Biochem 2001;11:61-76.. [4]. 34376558. Mechanosensitive channel gating by delipidation.. Flegler VJ, Rasmussen A, Borbil K, Boten L, Chen HA, Deinlein H,. Halang J, Hellmanzik K, Loffler J, Schmidt V, Makbul C, Kraft C,. Hedrich R, Rasmussen T, Bottcher B;. Proc Natl Acad Sci U S A. 2021; [Epub ahead of print]. [5]. 23012406. Conformational state of the MscS mechanosensitive channel in. solution revealed by pulsed electron-electron double resonance. (PELDOR) spectroscopy.. Pliotas C, Ward R, Branigan E, Rasmussen A, Hagelueken G, Huang. H, Black SS, Booth IR, Schiemann O, Naismith JH;. Proc Natl Acad Sci U S A. 2012;109:E2675-E2682.. [6]. 23339071. Open and shut: crystal structures of the dodecylmaltoside. solubilized mechanosensitive channel of small conductance from. Escherichia coli and Helicobacter pylori at 4.4 A and 4.1 A. resolutions.. TRUNCATED at 1650 bytes (from Pfam)

Date:

2024-04-03

Gene:

mscM

Date:

2021-08-26