Protein Family Models

Proteins containing this zinc ribbon domain at the C-terminus include the RNA-guided DNA endonuclease TnpB, found as an accessory protein (that is, not required for transposition) in many IS (insertion sequence) elements.

Date:

2024-08-14

This family includes IS891 [1], IS1136 [2] and IS1341 [3]. DUF1225, Pfam:PF06774, has now been merged into this family. [1]. 2553665. Characterization of an insertion sequence (IS891) of novel. structure from the cyanobacterium Anabaena sp. strain M-131.. Bancroft I, Wolk CP;. J Bacteriol 1989;171:5949-5954.. [2]. 8386127. IS1136, an insertion element in the erythromycin gene cluster of. Saccharopolyspora erythraea.. Donadio S, Staver MJ;. Gene 1993;126:147-151.. Sequence of IS1341. [3]. 7557457. A novel insertion sequence (IS)-like element of the thermophilic. bacterium PS3 promotes expression of the alanine carrier. protein-encoding gene.. Murai N, Kamata H, Nagashima Y, Yagisawa H, Hirata H;. gene 1995;163:103-107. (from Pfam)

Date:

2024-08-14

This family includes RNA-guided endonuclease TnpB from IS200/IS605 family elements (NF038281) and IS607 family elements (NF038280), but also many additional proteins. It exhibits homolog to or actually includes some CRISPR-associated (Cas) proteins such as the type V CRISPR-associated protein C2c8. For a long time, TnpB proteins were described as accessory proteins in IS (insertion sequence) elements, present as one of just one or two proteins encoded in the element but not necessary for transposition. The programmable RNA-guided endonuclease TnpB proteins may provide a CRISPR-like, widespread form of phage defense by RNA-guided DNA degradation.

Date:

2021-10-16