Warning: The NCBI web site requires JavaScript to function. more...
An official website of the United States government
The .gov means it's official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.
The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.
HNH endonuclease
WHH is a predicted nuclease of the HNH/ENDO VII superfamily of the treble clef fold. The name is derived from the conserved motif WHH. It is found in bacterial polymorphic toxin systems [1] and functions as a toxin module. WHH is the shortest version of HNH nuclease families. Like AHH and LHH, the WHH nuclease contains 4 conserved histidines of which the first one is predicted to bind a metal-ion and other three ones are involved in activation of water molecule for hydrolysis [1]. [1]. 21306995. A novel immunity system for bacterial nucleic acid degrading. toxins and its recruitment in various eukaryotic and DNA viral. systems.. Zhang D, Iyer LM, Aravind L;. Nucleic Acids Res. 2011;39:4532-4552. (from Pfam)
Colicin-like bacteriocins are complex structures with an N-terminal beta-barrel translocation domain (Pfam:PF09000), a long double-alpha-helical receptor-binding domain (Pfam:PF11570) and this C-terminal RNAse/DNase domain with endonuclease activity. Their competitor bacteriocidal action is by a process that involves binding to a surface receptor, entering the cell, and, finally, killing it. The lethal action of colicin E3 is a specific cleavage in the ribosomal decoding A site. The crystal structure of colicin E3 reveals a Y-shaped molecule with the receptor binding domain forming a 100 Angstrom long stalk and the two globular heads of the translocation domain and this catalytic domain comprising the two arms [2]. [1]. 10368275. The crystal structure of the DNase domain of colicin E7 in. complex with its inhibitor Im7 protein.. Ko TP, Liao CC, Ku WY, Chak KF, Yuan HS;. Structure. 1999;7:91-102.. [2]. 11741540. Crystal structure of colicin E3: implications for cell entry and. ribosome inactivation.. Soelaiman S, Jakes K, Wu N, Li C, Shoham M;. Mol Cell. 2001;8:1053-1062. (from Pfam)
Filter your results:
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on