Protein Family Models

This domain is functionally uncharacterised, found at the N-terminal of the uncharacterised UPF0758 proteins from bacteria and archaea, and is approximately 90 amino acids in length. UPF0758 was previously known as the radC family, a name that was assigned according to the radC102 mutant of E. coli which was later demonstrated to be an allele of the transcription-repair-coupling factor recG [1, 2]. UPF0758 has been described as a putative JAMM-family deubiquitinating enzyme, but its function remains to be determined [3]. Structure prediction using Colab notebook from AlphaFold DB suggests that it has an alpha bundle fold. It may contain two helix-hairpin-helix (HhH) motifs. This domain is found in association with Pfam:PF04002 [4]. [1]. 11053371. radC102 of Escherichia coli is an allele of recG.. Lombardo MJ, Rosenberg SM;. J Bacteriol. 2000;182:6287-6291.. [2]. 18556794. RadC, a misleading name?.. Attaiech L, Granadel C, Claverys JP, Martin B;. J Bacteriol. 2008;190:5729-5732.. [3]. 14737182. JAMM: a metalloprotease-like zinc site in the proteasome and. signalosome.. Ambroggio XI, Rees DC, Deshaies RJ;. PLoS Biol. 2004;2:E2.. [4]. 21890906. Evolution of the deaminase fold and multiple origins of. eukaryotic editing and mutagenic nucleic acid deaminases from. bacterial toxin systems.. Iyer LM, Zhang D, Rogozin IB, Aravind L;. Nucleic Acids Res. 2011; [Epub ahead of print] (from Pfam)

Date:

2024-04-03

A family of proteins present widely across the bacteria. This family was named initially with reference to the E. coli radC102 mutation which suggested that RadC was involved in repair of DNA lesions [1]. However the relevant mutation has subsequently been shown to be in recG, where radC is in fact an allele of recG [2]. In addition, a personal communication from Claverys, J-P, et al, indicates a total failure of all attempts to characterise a radiation-related function for RadC in Streptococcus pneumoniae, suggesting that it is not involved in repair of DNA lesions, in recombination during transformation, in gene conversion, nor in mismatch repair. Computational analysis, however, provides a possible function. The RadC-like family belong to the JAB superfamily of metalloproteins [3]. The domain shows fusions to an N-terminal Helix-hairpin-Helix (HhH) domain in most instances. Other domain combinations include fusions to the anti-restriction module ArdC, the DinG/RAD3-like superfamily II helicases and the DNAG-like primase. In some bacteria, closely related DinG/Rad3- like superfamily II helicases are fused to a 3'-5' exonuclease in the same position as the RadC-like JAB domain. These conserved domain associations lead to the hypothesis that the RadC-like JAB domains might function as a nuclease [3]. [1]. 10224240. Tandem repeat recombination induced by replication fork defects. in Escherichia coli requires a novel factor, RadC.. Saveson CJ, Lovett ST;. Genetics 1999;152:5-13.. [2]. 11053371. radC102 of Escherichia coli is an allele of recG.. Lombardo MJ, Rosenberg SM;. J Bacteriol. 2000;182:6287-6291.. [3]. 21890906. . TRUNCATED at 1650 bytes (from Pfam)

Date:

2024-04-03

The genes in this family for which the functions are known have an as yet porrly defined role in determining sensitivity to DNA damaging agents such as UV irradiation. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University).

Gene:

radC

Date:

2021-04-27

Gene:

radC

Date:

2021-07-22