Members of this family are asparaginyl peptidases [1]. The blood fluke parasite Schistosoma mansoni has at least five Clan CA cysteine peptidases in its digestive tract including cathepsins B (2 isoforms), C, F and L. All have been recombinantly expressed as active enzymes, albeit in various stages of activation [2]. In addition, a Clan CD peptidase, termed asparaginyl endopeptidase or 'legumain' has been identified. This has formerly been characterised as a 'haemoglobinase', but this term is probably incorrect [2]. Two cDNAs have been described for Schistosoma mansoni legumain; one encodes an active enzyme whereas the active site cysteine residue encoded by the second cDNA is substituted by an asparagine residue. Both forms have been recombinantly expressed [3]. [1]. 9065484. Cloning, isolation, and characterization of mammalian legumain,. an asparaginyl endopeptidase.. Chen JM, Dando PM, Rawlings ND, Brown MA, Young NE, Stevens RA,. Hewitt E, Watts C, Barrett AJ;. J Biol Chem 1997;272:8090-8098.. [2]. 15105025. Blood 'n' guts: an update on schistosome digestive peptidases.. Caffrey CR, McKerrow JH, Salter JP, Sajid M;. Trends Parasitol 2004;20:241-248.. [3]. 10682836. Identification of a cDNA encoding an active asparaginyl. endopeptidase of Schistosoma mansoni and its expression in. Pichia pastoris.. Caffrey CR, Mathieu MA, Gaffney AM, Salter JP, Sajid M, Lucas. KD, Franklin C, Bogyo M, McKerrow JH;. FEBS Lett 2000;466:244-248. (from Pfam)
GO Terms:- Date:
- 2024-08-14