Protein Family Models

This domain is found in a wide variety of contexts, but mostly occurring in cell wall associated proteins. A lack of conserved catalytic residues suggests that it is a binding domain. From context, possible substrates are hyaluronate or fibronectin (personal obs: C Yeats). This is further evidenced by [1]. Possibly the exact substrate is N-acetyl glucosamine. Finding it in the same protein as Pfam:PF05089 further supports this proposal. It is found in the C-terminal part of Swiss:O82833, which is removed during maturation ([2]). Some of the proteins it is found in (e.g. Swiss:Q9RL69) are involved in methicillin resistance ([3]). The name FIVAR derives from Found In Various Architectures. [1]. 12438356. Identification of a fibronectin-binding protein from. Staphylococcus epidermidis.. Williams RJ, Henderson B, Sharp LJ, Nair SP;. Infect Immun 2002;70:6805-6810.. [2]. 14759609. Posttranslational processing of polysaccharide lyase: maturation. route for gellan lyase in Bacillus sp. GL1.. Miyake O, Kobayashi E, Nankai H, Hashimoto W, Mikami B, Murata. K;. Arch Biochem Biophys 2004;422:211-220.. [3]. 10896508. Tn551-mediated insertional inactivation of the fmtB gene. encoding a cell wall-associated protein abolishes methicillin. resistance in Staphylococcus aureus.. Komatsuzawa H, Ohta K, Sugai M, Fujiwara T, Glanzmann P,. Berger-BachiB, Suginaka H;. J Antimicrob Chemother 2000;45:421-431. (from Pfam)

Date:

2024-08-14

The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively [1]. The transglycosylase domain catalyses the polymerisation of murein glycan chains ([4]). [1]. 9244263. Topographical and functional investigation of Escherichia coli. penicillin-binding protein 1b by alanine stretch scanning. mutagenesis.. F. Lefevre, M. H. Remy & J. M. Masson;. J Bacteriol 1997;179:4761-4767.. [2]. 9614972. X-ray studies of enzymes that interact with penicillins.. Kelly JA, Kuzin AP, Charlier P, Fonze E;. Cell Mol Life Sci 1998;54:353-358.. [3]. 8830253. Monofunctional biosynthetic peptidoglycan transglycosylases.. Spratt BG, Zhou J, Taylor M, Merrick MJ;. Mol Microbiol 1996;19:639-640.. [4]. 12867450. The glycosyltransferase domain of penicillin-binding protein 2a. from Streptococcus pneumoniae catalyzes the polymerization of. murein glycan chains.. Di Guilmi AM, Dessen A, Dideberg O, Vernet T;. J Bacteriol 2003;185:4418-4423. (from Pfam)

Date:

2024-08-14

The active site serine (residue 337 in Swiss:P14677) is conserved in all members of this family. [1]. 8605631. X-ray structure of Streptococcus pneumoniae PBP2x, a primary. penicillin target enzyme.. Pares S, Mouz N, Petillot Y, Hakenbeck R, Dideberg O. Nat Struct Biol 1996;3:284-289. (from Pfam)

Date:

2024-08-14