Protein Family Models

This family includes both TraG and TraD as well as VirD4 proteins. TraG is essential for DNA transfer in bacterial conjugation. These proteins are thought to mediate interactions between the DNA-processing (Dtr) and the mating pair formation (Mpf) systems [1]. This domain interacts with the relaxosome component TraM via the latter's tetramerisation domain. TraD is a hexameric ring ATPase that forms the cytoplasmic face of the conjugative pore [2]. [1]. 11976307. TraG-like proteins of DNA transfer systems and of the. Helicobacter pylori type IV secretion system: inner membrane. gate for exported substrates?. Schroder G, Krause S, Zechner EL, Traxler B, Yeo HJ, Lurz R,. Waksman G, Lanka E;. J Bacteriol 2002;184:2767-2779.. [2]. 18717787. Structural basis of specific TraD-TraM recognition during F. plasmid-mediated bacterial conjugation.. Lu J, Wong JJ, Edwards RA, Manchak J, Frost LS, Glover JN;. Mol Microbiol. 2008;70:89-99. (from Pfam)

Date:

2024-08-14

The plasmid conjugative coupling protein TrwB forms hexamers from six structurally very similar protomers [1]. This hexamer contains a central channel running from the cytosolic pole (made up by the AADs) to the membrane pole ending at the transmembrane pore shaped by 12 transmembrane helices, rendering an overall mushroom-like structure. The TrwB_AAD (all-alpha domain) domain appears to be the DNA-binding domain of the structure. TrwB, a basic integral inner-membrane nucleoside-triphosphate-binding protein, is the structural prototype for the type IV secretion system coupling proteins, a family of proteins essential for macromolecular transport between cells and export [2]. [1]. 11214325. The bacterial conjugation protein TrwB resembles ring helicases. and F1-ATPase.. Gomis-Ruth FX, Moncalian G, Perez-Luque R, Gonzalez A, Cabezon. E, de la Cruz F, Coll M;. Nature. 2001;409:637-641.. [2]. 11748238. Conjugative plasmid protein TrwB, an integral membrane type IV. secretion system coupling protein. Detailed structural features. and mapping of the active site cleft.. Gomis-Ruth FX, Moncalian G, de la Cruz F, Coll M;. J Biol Chem. 2002;277:7556-7566. (from Pfam)

Date:

2024-08-14

These proteins contain a P-loop and walker-B site for nucleotide binding. TraG is essential for DNA transfer in bacterial conjugation. These proteins are thought to mediate interactions between the DNA-processing (Dtr) and the mating pair formation (Mpf) systems [2]. The C-terminus of this domain interacts with the relaxosome component TraM via the latter's tetramerisation domain. TraD is a hexameric ring ATPase that forms the cytoplasmic face of the conjugative pore [3]. The family contains a number of different DNA transfer proteins [4]. [1]. 9767571. Sequence and analysis of the 60 kb conjugative,. bacteriocin-producing plasmid pMRC01 from Lactococcus lactis. DPC3147. Dougherty BA, Hill C, Weidman JF, Richardson DR, Venter JC, Ross. RP;. Mol Microbiol 1998;29:1029-1038.. [2]. 11976307. TraG-like proteins of DNA transfer systems and of the. Helicobacter pylori type IV secretion system: inner membrane. gate for exported substrates?. Schroder G, Krause S, Zechner EL, Traxler B, Yeo HJ, Lurz R,. Waksman G, Lanka E;. J Bacteriol 2002;184:2767-2779.. [3]. 18717787. Structural basis of specific TraD-TraM recognition during F. plasmid-mediated bacterial conjugation.. Lu J, Wong JJ, Edwards RA, Manchak J, Frost LS, Glover JN;. Mol Microbiol. 2008;70:89-99.. [4]. 17259614. Interaction between the co-inherited TraG coupling protein and. the TraJ membrane-associated protein of the H-plasmid. conjugative DNA transfer system resembles chromosomal DNA. translocases.. Gunton JE, Gilmour MW, Baptista KP, Lawley TD, Taylor DE;. Microbiology. 2007;153:428-441. (from Pfam)

Date:

2024-08-14

type IV secretory system conjugative DNA transfer family protein similar to Agrobacterium tumefaciens VirD4

Date:

2024-08-07