Our present understanding of the sequence and mechanisms of human genital organogenesis is reviewed. Current theories about the derivation of the vaginal epithelium are examined and tested against two anomalous circumstances, congenital androgen insensitivity and agenesis of the lower vagina, which are presented as examples demonstrating the respective participation of the urogenital sinus or of the Müllerian ducts alone in the developmental process. The abnormalities recently described in the vagina and cervix of girls exposed in utero to diethylstilbestrol (DES) correspond remarkably with those encountered in lower vaginal agenesis, particularly with regard to the presence of vaginal adenosis, the deficiency of glycogen in the squamous cells (squamous metaplasia), and the abnormal response of the squamous epithelium to Schiller's iodine test. It is concluded that the development of the human vagina is best explained by the theory which holds that the Müllerian ducts in fetal life extend caudally to the level of the future hymen. After fusion of these ducts, squamous cells arising in the epithelium of the urogenital sinus invade from below, advance, and replace completely the Müllerian mucosa up to the level of the external os of the cervical canal.
PIP: The course of development of the human genital tract is undifferentiated up to the 9th week (32 mm). At this time both Wolffian (mesonephric) and Mullerian (paramesonephric) ducts are present as symmetric paired structures. These, together with the urogenital sinus and the metanephric ducts, provide the tissue sources for the internal genital and urinary apparatus, exclusive of the gonads and kidneys. Configuration of the oviducts varies among species. Most human anomalies may be represented in other species so that some authors consider them to be atavistic reversions. The gonad of the developing male fetus plays a critical role in the formation of the genital tract. It elaborates androgenic steroids and a polypeptide, a Mullerian inhibiting substance, which induced suppression and resorotion of the Mullerian ducts. In the female the Mullerian ducts grow and develop into their adult morphology while the Wolffian ducts persist only as microscopic islands. The development of the external genitals and secondary sex characteristics depends upon further exposure to androgenic or estrogenic hormone milieu. a case is reported of an instance of congenital absence of the upper vagina. At laparotomy normal sized uterus, tubes, and ovaries were found. Further plastic surgery via the vagina corrected the condition. 15 years later (age 32) it was learned that she had been married and had 3 pregnancies. The adenosis, areas of squamous metaplasia, and deformities of the cervix of girls exposed in utero to diethylestibestrol are examples of deranged development. The shallow depth or absence of the vaginal canal of individuals with testicular feminization are also due to faulty development. Both Mullerian tissue and that of the urogenital sinus origin normally participate in the development of the vagina. In the normal adult the squamous cells that line the vagina contain abundant glycogen indicating urogenital origin. Glycogen-deficient squamous cells and adenosis are thought to be of Mullerian origin. In an accompanying discussion additional details of development are mentioned. It was noted that 7 cases of adenocarcinoma of the prostatic utricle in males have been reported as resembling endometrial carcinoma. The prostatic utricle is a homologue of the uterus and upper vagina and may be involved in similar deranged developments