Abstract
The tlrB gene, which confers inducible resistance to a range of macrolide antibiotics including biosynthetic precursors of tylosin, was isolated and sequenced. In the genome of Streptomyces fradiae, it lies between pbp, which encodes a putative penicillin-binding protein, and tylN, encoding a glycosyltransferase involved in tylosin biosynthesis. The TlrB protein was produced in E. coli as a fusion to MalE. The fusion protein, but not MalE alone, inactivates macrolides in the presence of S-adenosyl-methionine (SAM) but the modified product(s) has not been characterised.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
ATP-Binding Cassette Transporters*
-
Amino Acid Sequence
-
Anti-Bacterial Agents / biosynthesis*
-
Anti-Bacterial Agents / chemistry
-
Bacterial Proteins / chemistry*
-
Bacterial Proteins / genetics
-
Carrier Proteins / genetics
-
Colony Count, Microbial
-
DNA, Bacterial / analysis
-
DNA, Bacterial / genetics
-
Drug Resistance, Microbial
-
Escherichia coli Proteins*
-
Fermentation
-
Genes, Bacterial*
-
Genome
-
Maltose-Binding Proteins
-
Methyltransferases / metabolism
-
Molecular Sequence Data
-
Monosaccharide Transport Proteins*
-
Periplasmic Binding Proteins*
-
Phenotype
-
Recombinant Fusion Proteins / biosynthesis
-
Recombinant Fusion Proteins / pharmacology
-
Reverse Transcriptase Polymerase Chain Reaction
-
Streptomyces / enzymology
-
Streptomyces / genetics
-
Streptomyces / metabolism*
-
Tylosin / biosynthesis*
-
Tylosin / chemistry
Substances
-
ATP-Binding Cassette Transporters
-
Anti-Bacterial Agents
-
Bacterial Proteins
-
Carrier Proteins
-
DNA, Bacterial
-
Escherichia coli Proteins
-
MalE protein, E coli
-
Maltose-Binding Proteins
-
Monosaccharide Transport Proteins
-
Periplasmic Binding Proteins
-
Recombinant Fusion Proteins
-
maltose transport system, E coli
-
Methyltransferases
-
TlrB protein, Streptomyces fradiae
-
Tylosin