Abstract
The small adaptor protein RIL consists of two segments, the C-terminal LIM and the N-terminal PDZ domain, which mediate multiple protein-protein interactions. The RIL LIM domain can interact with PDZ domains in the protein tyrosine phosphatase PTP-BL and with the PDZ domain of RIL itself. Here, we describe and characterise the interaction of the RIL PDZ domain with the zyxin-related protein TRIP6, a protein containing three C-terminal LIM domains. The second LIM domain in TRIP6 is sufficient for a strong interaction with RIL. A weaker interaction with the third LIM domain in TRIP6, including the proper C-terminus, is also evident. TRIP6 also interacts with the second out of five PDZ motifs in PTP-BL. For this interaction to occur both the third LIM domain and the proper C-terminus are necessary. RNA expression analysis revealed overlapping patterns of expression for TRIP6, RIL and PTP-BL, most notably in tissues of epithelial origin. Furthermore, in transfected epithelial cells TRIP6 can be co-precipitated with RIL and PTP-BL PDZ polypeptides, and a co-localisation of TRIP6 and RIL with Factin structures is evident. Taken together, PTP-BL, RIL and TRIP6 may function as components of multi-protein complexes at actin-based sub-cellular structures.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATPases Associated with Diverse Cellular Activities
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Actins / metabolism
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Adaptor Proteins, Signal Transducing*
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Amino Acid Motifs
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Amino Acid Sequence
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Animals
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Blotting, Western
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Caco-2 Cells
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Cloning, Molecular
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Cytoskeletal Proteins
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / metabolism*
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Fluorescent Antibody Technique
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Gene Library
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Glycoproteins
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Humans
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In Situ Hybridization
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LIM Domain Proteins
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Metalloproteins / metabolism*
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Mice
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Microfilament Proteins
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Molecular Sequence Data
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Plasmids
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Precipitin Tests
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Proteasome Endopeptidase Complex
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Protein Binding
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Protein Structure, Tertiary
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Protein Tyrosine Phosphatase, Non-Receptor Type 13
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Protein Tyrosine Phosphatases / chemistry
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Protein Tyrosine Phosphatases / metabolism*
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RNA, Messenger / metabolism
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Sequence Homology, Amino Acid
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Tissue Distribution
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Transcription Factors / chemistry
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Transcription Factors / metabolism*
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Transfection
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Two-Hybrid System Techniques
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Zyxin
Substances
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Actins
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Adaptor Proteins, Signal Transducing
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Cytoskeletal Proteins
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DNA-Binding Proteins
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Glycoproteins
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LIM Domain Proteins
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Metalloproteins
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Microfilament Proteins
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PDLIM4 protein, human
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PSMC5 protein, human
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Pdlim4 protein, mouse
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RNA, Messenger
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Transcription Factors
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ZYX protein, human
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Zyx protein, mouse
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Zyxin
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PTPN13 protein, human
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Protein Tyrosine Phosphatase, Non-Receptor Type 13
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Protein Tyrosine Phosphatases
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Ptpn13 protein, mouse
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Proteasome Endopeptidase Complex
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ATPases Associated with Diverse Cellular Activities