Characterization of three isotypes of immunoglobulin light chains and T-cell antigen receptor alpha in zebrafish

Immunogenetics. 2000 Sep;51(11):915-23. doi: 10.1007/s002510000229.

Abstract

The zebrafish (Danio rerio) has become a significant model for understanding the developmental regulation of gene expression and holds considerable potential for characterizing the development of the immune system. Using a number of different approaches, including heterologous hybridization and short-primer PCR, cDNAs for three different classes of light-chain genes were identified and characterized. The zebrafish light chains are similar to trout type 1, trout type 2, and catfish type F, respectively. T-cell antigen receptor alpha (TCRalpha) was also identified and characterized. A high proportion of unusual transcripts including sterile transcripts, germline VJC transcripts, aberrant splice forms, and V-V transcripts were encountered in the immunoglobulin and TCR cDNAs examined. The light-chain and TCRalpha loci each consist of multiple families of V gene segments, apparent even from the small numbers of cDNAs of each isotype sequenced. The gene sequences reported provide an essential set of markers of both B- and T-cell lineages that will facilitate investigations of immune system development.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA, Complementary
  • Genes, T-Cell Receptor alpha*
  • Immunoglobulin Constant Regions / classification
  • Immunoglobulin Constant Regions / genetics*
  • Immunoglobulin Isotypes / classification
  • Immunoglobulin Isotypes / genetics
  • Immunoglobulin Joining Region / classification
  • Immunoglobulin Joining Region / genetics*
  • Immunoglobulin Light Chains / classification
  • Immunoglobulin Light Chains / genetics*
  • Immunoglobulin Variable Region / classification
  • Immunoglobulin Variable Region / genetics*
  • Molecular Sequence Data
  • Phylogeny
  • Sequence Homology, Amino Acid
  • Zebrafish / genetics*
  • Zebrafish / immunology

Substances

  • DNA, Complementary
  • Immunoglobulin Constant Regions
  • Immunoglobulin Isotypes
  • Immunoglobulin Joining Region
  • Immunoglobulin Light Chains
  • Immunoglobulin Variable Region

Associated data

  • GENBANK/AF246162
  • GENBANK/AF246163
  • GENBANK/AF246164
  • GENBANK/AF246165
  • GENBANK/AF246166
  • GENBANK/AF246167
  • GENBANK/AF246168
  • GENBANK/AF246169
  • GENBANK/AF246170
  • GENBANK/AF246171
  • GENBANK/AF246172
  • GENBANK/AF246173
  • GENBANK/AF246174
  • GENBANK/AF246175
  • GENBANK/AF246176
  • GENBANK/AF246177
  • GENBANK/AF246178
  • GENBANK/AF246179
  • GENBANK/AF246180
  • GENBANK/AF246181
  • GENBANK/AF246182
  • GENBANK/AF246183
  • GENBANK/AF246184
  • GENBANK/AF246185
  • GENBANK/AF246186
  • GENBANK/AF246187
  • GENBANK/AF246188
  • GENBANK/AF246189
  • GENBANK/AF246190
  • GENBANK/AF246191