OXA-35 is an OXA-10-related beta-lactamase from Pseudomonas aeruginosa

D Aubert; L Poirel; A B Ali; F W Goldstein; P Nordmann

doi:10.1093/jac/48.5.717

OXA-35 is an OXA-10-related beta-lactamase from Pseudomonas aeruginosa

J Antimicrob Chemother. 2001 Nov;48(5):717-21. doi: 10.1093/jac/48.5.717.

Authors

D Aubert¹, L Poirel, A B Ali, F W Goldstein, P Nordmann

Affiliation

¹ Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 Le Kremlin-Bicêtre, Paris, France.

PMID: 11679562
DOI: 10.1093/jac/48.5.717

Abstract

Pseudomonas aeruginosa clinical isolate PA35 is resistant to amino- and ureido-penicillins, has intermediate susceptibility to cefsulodin, cefepime and aztreonam, and is susceptible to imipenem and ceftazidime. Cloning and sequencing revealed a new beta-lactamase variant, OXA-35, sharing 96% amino acid identity with OXA-10. OXA-35 displays a restricted-substrate hydrolysis profile with improved hydrolysis of amoxicillin and cloxacillin compared with OXA-10. OXA-35 differs from derivatives OXA-19 and OXA-28 by one amino acid substitution and may be a progenitor of these OXA-13-like extended-spectrum beta-lactamases.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aged
Aged, 80 and over
Amino Acid Substitution / genetics
Anti-Bacterial Agents / pharmacology
Cloning, Molecular
Cloning, Organism
DNA Transposable Elements / genetics
Drug Resistance, Bacterial / genetics
Genes, Bacterial
Humans
Molecular Sequence Data
Pseudomonas aeruginosa / drug effects
Pseudomonas aeruginosa / enzymology*
Pseudomonas aeruginosa / genetics
Pseudomonas aeruginosa / isolation & purification
beta-Lactamases / chemistry*
beta-Lactamases / genetics
beta-Lactams

Substances

Anti-Bacterial Agents
DNA Transposable Elements
beta-Lactams
beta-lactamase OXA-10
beta-Lactamases

Associated data

GENBANK/AF315786