Clinical isolates of Enterobacteriaceae producing extended-spectrum beta-lactamases: prevalence of CTX-M-3 at a hospital in China

Antimicrob Agents Chemother. 2003 Feb;47(2):790-3. doi: 10.1128/AAC.47.2.790-793.2003.

Abstract

The prevalence of extended-spectrum beta-lactamase-producing strains was demonstrated in 5 of 44 (11.4%) Escherichia coli, 17 of 43 (39.5%) Klebsiella pneumoniae, 3 of 50 (6.0%) Enterobacter cloacae, and 2 of 25 (8.0%) Citrobacter freundii strains at a teaching hospital in China. Nineteen of these 27 strains expressed CTX-M-3 beta-lactamase (pI 8.6). A subset of the clinical isolates expressing the CTX-M-3 enzyme, tested by pulsed-field gel electrophoresis, revealed multiple clones. Five isolates expressed a novel enzyme, SHV-43 (pI 8.0), which had two substitutions (Leu113Phe and Thr149Ser) compared with SHV-1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • China
  • Electrophoresis, Gel, Pulsed-Field
  • Enterobacteriaceae / enzymology*
  • Humans
  • Microbial Sensitivity Tests
  • beta-Lactamases / drug effects
  • beta-Lactamases / genetics*
  • beta-Lactamases / isolation & purification*

Substances

  • Anti-Bacterial Agents
  • beta-lactamase CTX-M-13
  • beta-Lactamases