Low content of protein S29 in ribosomes of human lung cancer cell line a549: detected by two-dimensional electrophoresis

Zhi-Dong Zhou; Lei Bao; Ding-Gan Liu; Min-Qian Li; Yin-Zi Ge; Ying-Ling Huang; Wang-Yi Liu

doi:10.2174/0929866033408273

Low content of protein S29 in ribosomes of human lung cancer cell line a549: detected by two-dimensional electrophoresis

Protein Pept Lett. 2003 Feb;10(1):91-7. doi: 10.2174/0929866033408273.

Authors

Zhi-Dong Zhou¹, Lei Bao, Ding-Gan Liu, Min-Qian Li, Yin-Zi Ge, Ying-Ling Huang, Wang-Yi Liu

Affiliation

¹ State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China.

PMID: 12625830
DOI: 10.2174/0929866033408273

Abstract

This study revealed that the content of protein S29 in ribosomes of cancer cell line A549 was distinctly low (equivalent to about 30% of that of 2BS). The conclusion was acquired based on the ratios of spot volume of ribosomal protein S29 to that of several other ribosomal proteins (S29/L37a, S29/L38, S29/S27 and S29/S28) in the same gel plate. The possible biological roles of ribosomal protein S29 in malignant transformation and translation regulation are briefly discussed.

MeSH terms

Blotting, Northern
Cell Line
Electrophoresis, Gel, Two-Dimensional
Humans
Liver Neoplasms / metabolism
Lung / cytology
Lung Neoplasms / metabolism*
RNA / isolation & purification
Ribosomal Proteins / analysis*
Ribosomal Proteins / isolation & purification
Silver Staining / methods

Substances

Ribosomal Proteins
ribosomal protein S29
RNA