Mitochondrial DNA sequence analysis of the cytochrome oxidase subunit I and II genes, the ATPase9 gene, the NADH dehydrogenase ND4L and ND5 gene complex, and the glutaminyl, methionyl and arginyl tRNA genes from Trichophyton rubrum

Curr Genet. 1992 Sep;22(3):229-34. doi: 10.1007/BF00351730.

Abstract

In this paper, we present the nucleotide sequence of a 5248 bp-long region of the mitochondrial (mt) genome of the dermatophyte Trichophyton rubrum. This region which represents about 1/4 of the total mt genome of this species reveals a compact organization of genes including: the glutaminyl tRNA, the methionyl tRNA, the cytochrome oxidase subunit I gene, the arginyl tRNA, the mitochondrial version of the ATPase subunit 9 gene, the cytochrome oxidase subunit II gene and a part of the NADH dehydrogenase ND4L and ND5 gene "complex". The main features of the part of mt DNA sequenced is the non-interrupted COXI gene and the presence in the mitochondrial version of the ATPase 9 gene of a small group IA intron. The extensive amino-acid sequence similarity with the equivalent gene in Aspergillus nidulans and Neuropora crassa indicates that this gene codes for a dicyclohexylcarbodiimide binding protein. The conserved arrangement of this portion of the mt genome and the presence of tRNAs between the protein-coding genes are compatible with a large polycistronic transcript processed by the excision of tRNAs, or similar secondary structures, as proposed for other fungal or mammalian mt DNAS.

MeSH terms

  • Adenosine Triphosphatases / genetics*
  • Amino Acid Sequence
  • Base Sequence
  • DNA, Fungal / genetics
  • DNA, Mitochondrial / genetics*
  • Electron Transport Complex IV / genetics*
  • Genes, Fungal
  • Introns
  • Molecular Sequence Data
  • NADH Dehydrogenase / genetics*
  • Nucleic Acid Conformation
  • RNA, Fungal / genetics
  • RNA, Transfer, Amino Acyl / genetics*
  • RNA, Transfer, Met / genetics
  • Sequence Homology, Nucleic Acid
  • Trichophyton / enzymology
  • Trichophyton / genetics*

Substances

  • DNA, Fungal
  • DNA, Mitochondrial
  • RNA, Fungal
  • RNA, Transfer, Amino Acyl
  • RNA, Transfer, Met
  • NADH Dehydrogenase
  • Electron Transport Complex IV
  • Adenosine Triphosphatases

Associated data

  • GENBANK/S45004
  • GENBANK/S45006
  • GENBANK/S45007
  • GENBANK/S72771
  • GENBANK/X65223
  • GENBANK/X65478
  • GENBANK/X65479
  • GENBANK/X65480
  • GENBANK/X65481
  • GENBANK/X65482