Elongation factor-1 messenger-RNA levels in cultured cells are high compared to tissue and are not drastically affected further by oncogenic transformation

Nucleic Acids Res. 1992 Nov 25;20(22):5907-10. doi: 10.1093/nar/20.22.5907.

Abstract

Copy-DNA clones covering the complete coding sequence of human Elongation Factor-1 gamma mRNA have been isolated and characterized. The expression of Elongation Factor-1 in a variety of cell lines and a number of tissues shows a large increase in Elongation Factor-1 mRNA going from tissue to cultured cells (20-fold). Messenger-RNA levels for Elongation Factor-1 alpha, -1 beta and -1 gamma increase in parallel suggesting coordinate regulation of the expression of these genes. Oncogenic transformation in vitro does not strongly affect Elongation Factor-1 mRNA levels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Cell Line, Transformed
  • Cell Transformation, Neoplastic / genetics*
  • Cells, Cultured
  • Humans
  • Molecular Sequence Data
  • Oncogenes
  • Organ Specificity / genetics
  • Peptide Elongation Factor 1
  • Peptide Elongation Factors / genetics*
  • Peptide Elongation Factors / metabolism
  • Polymerase Chain Reaction
  • RNA, Messenger / genetics*
  • Rats
  • Sequence Homology, Amino Acid

Substances

  • Peptide Elongation Factor 1
  • Peptide Elongation Factors
  • RNA, Messenger

Associated data

  • GENBANK/Z11531