Murine tenascin-W: a novel mammalian tenascin expressed in kidney and at sites of bone and smooth muscle development

J Cell Sci. 2004 Feb 1;117(Pt 4):571-81. doi: 10.1242/jcs.00867. Epub 2004 Jan 6.

Abstract

We cloned and characterized a novel member of the tenascin family of extracellular matrix proteins--the murine orthologue of zebrafish tenascin-W. Full-length recombinant tenascin-W was expressed and purified from mammalian cell cultures. Rotary shadowing followed by electron microscopy showed that tenascin-W forms hexabrachions. We studied its expression during development and in the adult by immunohistochemistry, in situ hybridization and immunoblotting. Tenascin-W is expressed during palate formation, osteogenesis and smooth muscle development. In the adult, tenascin-W is found in the kidney, cardiac semilunar valves, corneal limbus and periosteum. Tenascin-W and tenascin-C expression overlap in many of these areas. Bone-morphogenic-protein-2 treated C2C12 cells secrete tenascin-W and are able to adhere to and to extend actin-rich processes on a tenascin-W substratum. In vitro, cells bind to tenascin-W in an RGD-dependent manner. This adhesion is increased by transfection of alpha8 integrin, which localizes with tenascin-W in the periosteum and kidney.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Bone and Bones / embryology
  • Bone and Bones / metabolism*
  • Cell Adhesion / physiology
  • Cells, Cultured
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • DNA, Complementary / isolation & purification
  • Gene Expression Regulation, Developmental*
  • Humans
  • Immunohistochemistry
  • In Situ Hybridization
  • Kidney / embryology
  • Kidney / metabolism*
  • Mice
  • Molecular Sequence Data
  • Muscle, Smooth / embryology
  • Muscle, Smooth / metabolism*
  • Organ Specificity
  • Phylogeny
  • Recombinant Proteins / genetics
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Tenascin / analogs & derivatives
  • Tenascin / biosynthesis
  • Tenascin / genetics*
  • Tenascin / isolation & purification
  • Zebrafish
  • Zebrafish Proteins / genetics

Substances

  • DNA, Complementary
  • Recombinant Proteins
  • Tenascin
  • Zebrafish Proteins
  • tenascin-N protein, mouse
  • tnn protein, zebrafish

Associated data

  • GENBANK/AJ580920