Morphine modulates HIV-1 gp160-induced murine macrophage and human monocyte apoptosis by disparate ways

J Neuroimmunol. 2004 Mar;148(1-2):86-96. doi: 10.1016/j.jneuroim.2003.11.015.

Abstract

We studied the effect of HIV-1 gp160 protein and morphine on murine macrophage and human monocyte apoptosis. gp160 not only promoted murine macrophage apoptosis but also enhanced macrophage iNOS expression/NO generation. gp160 also altered macrophage bax and bcl-2 expression. Morphine enhanced (P<0.001) the effect of gp160 on macrophage apoptosis as well as iNOS expression/NO generation. Nevertheless, both morphine- and gp160-induced murine macrophage apoptosis was attenuated by nitric oxide synthase (NOS) inhibitors (L-NAME and L-NMMA). On the other hand, free radical scavengers such as superoxide dismutase (SOD), dimethylthiourea (DMTU) and catalase attenuated morphine and gp160-induced human monocyte apoptosis.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Analysis of Variance
  • Animals
  • Antibodies / pharmacology
  • Apoptosis*
  • Blotting, Southern / methods
  • Blotting, Western / methods
  • CD4 Antigens / immunology
  • Caspase 3
  • Caspases / metabolism
  • Catalase / pharmacology
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Drug Interactions
  • Enzyme Inhibitors / pharmacology
  • Enzyme-Linked Immunosorbent Assay / methods
  • HIV Envelope Protein gp160 / pharmacology*
  • Humans
  • In Situ Nick-End Labeling / methods
  • Lipopolysaccharides / pharmacology
  • Macrophages / drug effects*
  • Macrophages / physiology
  • Mice
  • Monocytes / drug effects*
  • Monocytes / physiology
  • Morphine / pharmacology*
  • NG-Nitroarginine Methyl Ester / pharmacology
  • Narcotics / pharmacology*
  • Nitric Oxide / metabolism
  • Proto-Oncogene Proteins / metabolism
  • Proto-Oncogene Proteins c-bcl-2 / metabolism
  • Species Specificity
  • Superoxide Dismutase / pharmacology
  • Thiourea / analogs & derivatives*
  • Thiourea / pharmacology
  • bcl-2-Associated X Protein

Substances

  • Antibodies
  • BAX protein, human
  • Bax protein, mouse
  • CD4 Antigens
  • Enzyme Inhibitors
  • HIV Envelope Protein gp160
  • Lipopolysaccharides
  • Narcotics
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • bcl-2-Associated X Protein
  • Nitric Oxide
  • Morphine
  • 1,3-dimethylthiourea
  • Catalase
  • Superoxide Dismutase
  • CASP3 protein, human
  • Casp3 protein, mouse
  • Caspase 3
  • Caspases
  • Thiourea
  • NG-Nitroarginine Methyl Ester