Structure of the capsular polysaccharide of Actinobacillus pleuropneumoniae serotype 5b

Eur J Biochem. 1992 Feb 15;204(1):225-30. doi: 10.1111/j.1432-1033.1992.tb16628.x.

Abstract

The capsular polysaccharide of Actinobacillus pleuropneumoniae serotype 5b (strain L20) was found to be a high molecular mass polymer composed of 2-acetamido-2-deoxy-D-glucose, D-glucose, and 3-deoxy-D-manno-octulosonic acid (KDO). Methylation analysis, partial hydrolysis and a combination of homonuclear and 1H-detected heteronuclear shift-correlated nuclear magnetic resonance experiments showed the polysaccharide to be a branched polymer of a trisaccharide repeating unit, having the structure: [formula; see text]

MeSH terms

  • Actinobacillus pleuropneumoniae / chemistry*
  • Bacterial Capsules / chemistry*
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Hydrolysis
  • Magnetic Resonance Spectroscopy
  • Methylation
  • Molecular Sequence Data
  • Molecular Structure
  • Molecular Weight
  • Polysaccharides, Bacterial / chemistry*

Substances

  • Polysaccharides, Bacterial