Abstract
Whether coat proteins play a widespread role in endocytic recycling remains unclear. We find that ACAP1, a GTPase-activating protein (GAP) for ADP-ribosylation factor (ARF) 6, is part of a novel clathrin coat complex that is regulated by ARF6 for endocytic recycling in two key physiological settings, stimulation-dependent recycling of integrin that is critical for cell migration and insulin-stimulated recycling of glucose transporter type 4 (Glut4), which is required for glucose homeostasis. These findings not only advance a basic understanding of an early mechanistic step in endocytic recycling but also shed key mechanistic insights into major physiological events for which this transport plays a critical role.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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3T3-L1 Cells
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ADP-Ribosylation Factor 6
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ADP-Ribosylation Factors / metabolism
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Amino Acid Sequence
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Animals
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Cell Membrane / metabolism
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Cell Membrane / ultrastructure
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Clathrin / genetics
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Clathrin / metabolism*
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Coat Protein Complex I / genetics
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Coat Protein Complex I / metabolism*
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Endocytosis / physiology*
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Endosomes / metabolism*
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Endosomes / ultrastructure
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GTPase-Activating Proteins / genetics
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GTPase-Activating Proteins / metabolism*
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Glucose Transporter Type 4 / genetics
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Glucose Transporter Type 4 / metabolism
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Humans
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Integrins / metabolism
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Mice
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Molecular Sequence Data
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Multiprotein Complexes
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Sequence Alignment
Substances
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ACAP1 protein, human
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ADP-Ribosylation Factor 6
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Clathrin
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Coat Protein Complex I
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GTPase-Activating Proteins
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Glucose Transporter Type 4
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Integrins
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Multiprotein Complexes
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ADP-Ribosylation Factors
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ARF6 protein, human
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Arf6 protein, mouse