Abstract
We have cloned a novel KRAB-related zinc finger gene, ZNF307, encoding a protein of 545aa. ZNF307 is conserved across species in evolution and is differentially expressed in human adult and fetal tissues. The fusion protein of EGFP-ZNF307 localizes in the nucleus. Transcriptional activity assays show ZNF307 suppresses transcriptional activity of L8G5-luciferase. Overexpressing ZNF307 in different cell lines also inhibits the transcriptional activities of p53 and p21. Moreover, ZNF307 works by reducing the p53 protein level and p53 protein reduction is achieved by increasing transcription of MDM2 and EP300. ZNF307 might suppress p53-p21 pathway through activating MDM2 and EP300 expression and inducing p53 degradation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Line
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Chlorocebus aethiops
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DNA-Binding Proteins / chemistry*
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Down-Regulation*
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E1A-Associated p300 Protein / genetics
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Evolution, Molecular
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Gene Expression Regulation*
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Genes, Reporter / genetics
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Humans
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Nuclear Proteins / metabolism
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Organ Specificity
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Proto-Oncogene Proteins c-mdm2 / genetics
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Proto-Oncogene Proteins p21(ras) / antagonists & inhibitors*
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Proto-Oncogene Proteins p21(ras) / metabolism
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Transcription, Genetic / genetics
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Transcriptional Activation / genetics
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Tumor Suppressor Protein p53 / antagonists & inhibitors*
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Tumor Suppressor Protein p53 / genetics
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Tumor Suppressor Protein p53 / metabolism
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Up-Regulation
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Zinc Fingers* / genetics
Substances
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DNA-Binding Proteins
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Nuclear Proteins
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Tumor Suppressor Protein p53
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ZKSCAN4 protein, human
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E1A-Associated p300 Protein
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EP300 protein, human
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Proto-Oncogene Proteins c-mdm2
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Proto-Oncogene Proteins p21(ras)