Abstract
The three gonadotropin (GtH) subunit cDNAs, GtHalpha, FSHbeta and LHbeta, which contain complete open reading frames were isolated from Southern catfish (Silurus meridionalis Chen) ovary. RT-PCR revealed that GtHalpha, FSHbeta and LHbeta mRNA were expressed in ovary, female and male pituitaries, but not in testis. Ontogeny study showed that GtHalpha and FSHbeta expressed in ovary from 25 dah (days after hatching) and LHbeta expressed from 40 dah onwards. The expression levels of these genes in all-female Southern catfish gonad were down-regulated after treatment with tamoxifen from 5 to 25 dah when measured at 65 dah. These results indicated the involvement of the three subunits in gonadal development and sexual differentiation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Catfishes / genetics
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Catfishes / metabolism*
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Female
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Follicle Stimulating Hormone, beta Subunit / chemistry
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Follicle Stimulating Hormone, beta Subunit / genetics
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Follicle Stimulating Hormone, beta Subunit / metabolism
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Gene Expression Profiling
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Gene Expression Regulation, Developmental / drug effects
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Gonadotropins / chemistry
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Gonadotropins / genetics
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Gonadotropins / metabolism*
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Gonads / cytology
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Gonads / drug effects
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Gonads / embryology*
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Gonads / metabolism*
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Luteinizing Hormone, beta Subunit / chemistry
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Luteinizing Hormone, beta Subunit / genetics
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Luteinizing Hormone, beta Subunit / metabolism
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Male
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Molecular Sequence Data
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Protein Subunits / chemistry
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Protein Subunits / genetics
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Protein Subunits / metabolism*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Sequence Alignment
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Sequence Analysis, Protein
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Tamoxifen / pharmacology
Substances
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Follicle Stimulating Hormone, beta Subunit
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Gonadotropins
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Luteinizing Hormone, beta Subunit
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Protein Subunits
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RNA, Messenger
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Tamoxifen
Associated data
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GENBANK/AY973945
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GENBANK/AY973946
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GENBANK/AY973947