The effect of doxorubicin on the glutathione content and viability of cultured human lung cancer cell lines A549 and GLC4 210

M al-Kabban; M J Stewart; I D Watson; J Reglinski

doi:10.1016/0009-8981(90)90127-e

The effect of doxorubicin on the glutathione content and viability of cultured human lung cancer cell lines A549 and GLC4 210

Clin Chim Acta. 1990 Dec 24;194(2-3):121-9. doi: 10.1016/0009-8981(90)90127-e.

Authors

M al-Kabban¹, M J Stewart, I D Watson, J Reglinski

Affiliation

¹ Kufa College of Medicine, Kufa University, Republic of Iraq.

PMID: 1965536
DOI: 10.1016/0009-8981(90)90127-e

Abstract

Glutathione was measured in doxorubicin-sensitive cells from small cell carcinoma of lung (GLC4 210), and the levels compared with those of cells with acquired resistance and a line of resistant non-small-cell adenocarcinoma A549 (Alveolar type 2). The effect of different doxorubicin concentrations on glutathione were measured by HPLC. The effect of doxorubicin on the viability of the cell lines was studied using thiazole blue dye reduction. An increase in A549 sensitivity to doxorubicin was produced using buthionine-S,R-sulfoximine at a non-toxic dose.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Adenocarcinoma / metabolism
Adenocarcinoma / pathology
Buthionine Sulfoximine
Carcinoma, Small Cell / metabolism
Carcinoma, Small Cell / pathology
Cell Survival / drug effects
Chromatography, High Pressure Liquid
Dose-Response Relationship, Drug
Doxorubicin / administration & dosage
Doxorubicin / pharmacology*
Glutathione / metabolism*
Humans
Lung Neoplasms / metabolism*
Lung Neoplasms / pathology
Methionine Sulfoximine / analogs & derivatives
Methionine Sulfoximine / pharmacology
Tumor Cells, Cultured

Substances

Methionine Sulfoximine
Buthionine Sulfoximine
Doxorubicin
Glutathione