Genes exclusively expressed in male germ cells encode proteins that play important roles in spermatogenesis and fertilization. In this study, we investigated the expression of a novel spermatogenic cell-specific gene known as amyotrophic lateral sclerosis 2 chromosome region candidate 12 (Als2cr12). Our in silico and in vitro analyses revealed that the mouse Als2cr12 gene produces two transcript isoforms by alternative splicing and that one of the isoforms is unique to spermatogenic cells. Using an antibody against the ALS2CR12 protein, we found that a protein from the germ cell-specific Als2cr12 transcript is present in mature sperm from the epididymis as well as germ cells in the testis. Further analysis of the ALS2CR12 protein in sperm disclosed the localization of the protein in the sperm tail. Specifically, our data suggest that the ALS2CR12 protein is associated with the fibrous sheath in the sperm flagellum. Thus, our study provides the first information regarding the expression of the Als2cr12 gene at the transcriptional, protein and cellular levels.
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