Abstract
Analysis of two clonally related multiresistant Pseudomonas aeruginosa isolates led to the identification of a novel IMP-type metallo-β-lactamase. IMP-29 was significantly different from the other IMP variants (the closest variant being IMP-5 with 93% amino acid identity). The bla(IMP-29) gene cassette was carried by a class 1 integron in strain 10.298, while in strain 10.266 it was located in a rearranged DNA region on a 30-kb conjugative plasmid. Biochemical analysis confirmed that IMP-29 efficiently hydrolyzed carbapenems.
Publication types
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Case Reports
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Research Support, Non-U.S. Gov't
MeSH terms
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Aged
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Anti-Bacterial Agents / therapeutic use
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Carbapenems / metabolism
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DNA, Bacterial / genetics
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Escherichia coli / drug effects
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Escherichia coli / genetics
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Fatal Outcome
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Humans
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Hydrolysis
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Integrons / genetics
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Male
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Microbial Sensitivity Tests
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Polymerase Chain Reaction
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Pseudomonas Infections / drug therapy
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Pseudomonas Infections / microbiology
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Pseudomonas aeruginosa / enzymology*
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Pseudomonas aeruginosa / genetics*
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Shock, Septic / drug therapy
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Shock, Septic / etiology
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beta-Lactamases / genetics*
Substances
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Anti-Bacterial Agents
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Carbapenems
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DNA, Bacterial
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beta lactamase IMP-29, Pseudomonas aeruginosa
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beta-Lactamases