Catalytic activity of APOBEC3F is required for efficient restriction of Vif-deficient human immunodeficiency virus

Virology. 2014 Feb:450-451:49-54. doi: 10.1016/j.virol.2013.11.041. Epub 2013 Dec 20.

Abstract

APOBEC3 proteins are DNA cytosine deaminases that restrict the replication of human immunodeficiency virus deficient in the counterdefense protein Vif. Here, we address the capacity of APOBEC3F to restrict via deaminase-dependent and -independent mechanisms by monitoring spreading infections in diverse T cell lines. Our data indicate that only a deaminase-proficient protein is capable of long-term restriction of Vif-deficient HIV in T cells, analogous to prior reports for APOBEC3G. This indicates that the principal mechanism of APOBEC3F restriction is deaminase-dependent.

Keywords: APOBEC3F; APOBEC3G; Deaminase; HIV restriction; Vif.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • APOBEC-3G Deaminase
  • Biocatalysis
  • Cell Line
  • Cytidine Deaminase / genetics
  • Cytidine Deaminase / metabolism
  • Cytosine Deaminase / chemistry*
  • Cytosine Deaminase / genetics
  • Cytosine Deaminase / metabolism*
  • Gene Products, vif / genetics
  • Gene Products, vif / metabolism*
  • HIV Infections / enzymology*
  • HIV Infections / virology
  • HIV-1 / genetics
  • HIV-1 / metabolism*
  • Humans
  • Protein Binding
  • T-Lymphocytes / enzymology
  • T-Lymphocytes / virology
  • vif Gene Products, Human Immunodeficiency Virus

Substances

  • Gene Products, vif
  • vif Gene Products, Human Immunodeficiency Virus
  • APOBEC3F protein, human
  • Cytosine Deaminase
  • APOBEC-3G Deaminase
  • APOBEC3G protein, human
  • Cytidine Deaminase