The WNT inhibitor APCDD1 sustains the expression of β-catenin during the osteogenic differentiation of human dental follicle cells

S Viale-Bouroncle; C Klingelhöffer; T Ettl; C Morsczeck

doi:10.1016/j.bbrc.2014.12.107

The WNT inhibitor APCDD1 sustains the expression of β-catenin during the osteogenic differentiation of human dental follicle cells

Biochem Biophys Res Commun. 2015 Feb 13;457(3):314-7. doi: 10.1016/j.bbrc.2014.12.107. Epub 2015 Jan 12.

Authors

S Viale-Bouroncle¹, C Klingelhöffer¹, T Ettl¹, C Morsczeck²

Affiliations

¹ Department of Cranio- and Maxillofacial Surgery, Hospital of the University of Regensburg, Regensburg, Germany.
² Department of Cranio- and Maxillofacial Surgery, Hospital of the University of Regensburg, Regensburg, Germany. Electronic address: christian.morsczeck@ukr.de.

PMID: 25592970
DOI: 10.1016/j.bbrc.2014.12.107

Abstract

In hair follicle cells APCDD1 inhibits the canonical WNT/β-Catenin pathway and its inactivation is associated with an autosomal dominant form of hair loss. We analyzed the role of APCDD1 for the osteogenic differentiation in dental follicle cells (DFCs) and identified a new and surprising function. Contrarily to hair follicle cells APCDD1 was crucial for the expression of β-Catenin and for the activity of the TCF/LEF reporter assay in DFCs. In addition, a depletion of APCDD1 inhibits the expression of osteogenic markers such as RUNX2 and decreased the matrix mineralization. However, similar to hair follicle cells in previous studies a control cell culture with oral squamous carcinoma cells showed that APCDD1 inhibits the expression of β-Catenin and of typical target genes of the canonical WNT/β-Catenin pathway. In conclusion, our data disclosed an unusual role of APCDD1 in DFCs during the osteogenic differentiation. APCDD1 sustains the expression and activation of β-Catenin.

Keywords: APCDD1; Dental stem cells; Osteogenic differentiation; Signaling pathway; WNT-signaling.

MeSH terms

Cell Differentiation / genetics
Cell Differentiation / physiology
Cells, Cultured
Core Binding Factor Alpha 1 Subunit / genetics
Dental Sac / cytology*
Dental Sac / metabolism*
Early Growth Response Protein 1 / genetics
Gene Expression
Homeodomain Proteins / genetics
Humans
Intracellular Signaling Peptides and Proteins / antagonists & inhibitors
Intracellular Signaling Peptides and Proteins / genetics
Intracellular Signaling Peptides and Proteins / metabolism*
Membrane Proteins / antagonists & inhibitors
Membrane Proteins / genetics
Membrane Proteins / metabolism*
Mesenchymal Stem Cells / cytology
Mesenchymal Stem Cells / metabolism
Osteogenesis / genetics
Osteogenesis / physiology
RNA, Small Interfering / genetics
Transcription Factors / genetics
Wnt Signaling Pathway
beta Catenin / genetics*

Substances

APCDD1 protein, human
CTNNB1 protein, human
Core Binding Factor Alpha 1 Subunit
Distal-less homeobox proteins
EGR1 protein, human
Early Growth Response Protein 1
Homeodomain Proteins
Intracellular Signaling Peptides and Proteins
Membrane Proteins
RNA, Small Interfering
RUNX2 protein, human
Transcription Factors
beta Catenin