Reidentification of Facultatively Alkaliphilic Bacillus sp. C-125 to Bacillus halodurans

H Takami; K Horikoshi

doi:10.1271/bbb.63.943

Reidentification of Facultatively Alkaliphilic Bacillus sp. C-125 to Bacillus halodurans

Biosci Biotechnol Biochem. 1999;63(5):943-5. doi: 10.1271/bbb.63.943.

Authors

H Takami¹, K Horikoshi¹

Affiliation

¹ a Deep-sea Microorganisms Research Group, Japan Marine Science and Technology Center.

PMID: 27385575
DOI: 10.1271/bbb.63.943

Abstract

Alkaliphilic Bacillus sp. C-125 was taxonomically characterized by physiological and biochemical characteristics, 16S rDNA sequence similarity, and DNA-DNA hybridization analyses. Phylogenetic analysis of strain C-125 based on comparison of 16S rDNA sequences showed that this strain is closely related to Bacillus halodurans. DNA-DNA hybridization analysis was done comparing C-125 and related Bacillus reference strains. The highest level of DNA-DNA relatedness (86%) was found between strain C-125 and B. halodurans. Our findings demonstrate that strain C-125 is a member of the species B. halodurans.

Keywords: DNA-DNA hybridization; alkaliphilic Bacillus halodurans C-125; identification; phylogenetic tree.