Molecular Epidemiology of Agents of Human Chromoblastomycosis in Brazil with the Description of Two Novel Species

Renata R Gomes; Vania A Vicente; Conceição M P S de Azevedo; Claudio G Salgado; Moises B da Silva; Flávio Queiroz-Telles; Sirlei G Marques; Daniel W C L Santos; Tania S de Andrade; Elizabeth H Takagi; Katia S Cruz; Gheniffer Fornari; Rosane C Hahn; Maria L Scroferneker; Rachel B Caligine; Mauricio Ramirez-Castrillon; Daniella P de Araújo; Daiane Heidrich; Arnaldo L Colombo; G S de Hoog

doi:10.1371/journal.pntd.0005102

Molecular Epidemiology of Agents of Human Chromoblastomycosis in Brazil with the Description of Two Novel Species

PLoS Negl Trop Dis. 2016 Nov 28;10(11):e0005102. doi: 10.1371/journal.pntd.0005102. eCollection 2016 Nov.

Authors

Renata R Gomes^{1

2}, Vania A Vicente¹, Conceição M P S de Azevedo³, Claudio G Salgado⁴, Moises B da Silva⁴, Flávio Queiroz-Telles^{1

5}, Sirlei G Marques^{6

7}, Daniel W C L Santos⁸, Tania S de Andrade⁹, Elizabeth H Takagi⁹, Katia S Cruz¹⁰, Gheniffer Fornari¹, Rosane C Hahn¹¹, Maria L Scroferneker¹², Rachel B Caligine¹³, Mauricio Ramirez-Castrillon¹⁴, Daniella P de Araújo⁴, Daiane Heidrich¹⁵, Arnaldo L Colombo⁸, G S de Hoog^{1

16}

Affiliations

¹ Microbiology, Parasitology and Pathology Post-graduation Program, Department of Basic Pathology, Federal University of Paraná, Curitiba, PR, Brazil.
² Department of Biological Science, State University of Parana/ Campus Paranaguá, Paranaguá, PR, Brazil.
³ Department of Medicine, Federal University of Maranhão, Sao Luis, MA, Brazil.
⁴ Dermato-Immunology Laboratory, Institute of Biological Sciences, Federal University of Para. Marituba, PA, Brazil.
⁵ Clinical Hospital of the Federal University of Paraná, Curitiba, PR, Brazil.
⁶ University Hospital of Federal University of Maranhão, Sao Luis, MA, Brazil.
⁷ Cedro Laboratories Maranhão, Sao Luis, MA, Brazil.
⁸ Division of Infectious Diseases, Federal University of São Paulo, SP, Brazil.
⁹ Department of Culture Collection, Adolfo Lutz Institute, São Paulo, SP, Brazil.
¹⁰ National Institute of Amazonian Research, Manaus, Brazil.
¹¹ Veterinary Laboratory of Molecular Biology, Faculty of Agronomy and Veterinary Medicine, Federal University of Mato Grosso, Cuiabá, MT, Brazil.
¹² Department of Microbiology, ICBS, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
¹³ Postgraduate Program in Medicine and Biomedicine, Santa Casa de Belo Horizonte Hospital, MG, Brazil.
¹⁴ Postgraduate Program in Cellular and Molecular Biology, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
¹⁵ Postgraduate Program in Medicine, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
¹⁶ Centraalbureau voor Schimmelcultures KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands.

Abstract

The human mutilating disease chromoblastomycosis is caused by melanized members of the order Chaetothyriales. To assess population diversity among 123 clinical strains of agents of the disease in Brazil we applied sequencing of the rDNA internal transcribed spacer region, and partial cell division cycle and β-tubulin genes. Strains studied were limited to three clusters divided over the single family Herpotrichiellaceae known to comprise agents of the disease. A Fonsecaea cluster contained the most important agents, among which F. pedrosoi was prevalent with 80% of the total set of strains, followed by 13% for F. monophora, 3% for F. nubica, and a single isolate of F. pugnacius. Additional agents, among which two novel species, were located among members of the genus Rhinocladiella and Cyphellophora, with frequencies of 3% and 1%, respectively.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ascomycota / classification
Ascomycota / genetics
Ascomycota / isolation & purification*
Brazil / epidemiology
Chromoblastomycosis / epidemiology
Chromoblastomycosis / microbiology*
DNA, Fungal / genetics
DNA, Ribosomal Spacer / genetics
Humans
Molecular Epidemiology
Mycological Typing Techniques
Phylogeny

Substances

DNA, Fungal
DNA, Ribosomal Spacer

Grants and funding

This work was supported by Brazilian government by financial support (Special Visiting Researcher Project; grant number 059/2012PVE-CAPES) from the Brazilian Federal Agency for Support and Evaluation of Graduate: Education Coordination for the Improvement of Higher Education Personnel—CAPES (www.capes.gov.br) with fellowship for RRG, GH and GSdH. The authors VAV and CGS were supported by fellowship from National Counsel of Technological and Scientific Development (http://cnpq.br/), Brasilia, Brazil. The author DPA received fellowship from Fundação Amazônia de Amparo a Estudos e Pesquisas- FAPESPA (http://www.fapespa.pa.gov.br) and CGS received financial support from CAPES /PROAMAZONIA grant number 3288/2013. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.