Calnexin promotes the folding of mutant iduronate 2-sulfatase related to mucopolysaccharidosis type II

Biochem Biophys Res Commun. 2019 Jun 18;514(1):217-223. doi: 10.1016/j.bbrc.2019.04.115. Epub 2019 Apr 24.

Abstract

Mucopolysaccharidosis type II (MPS II) is one of the most common mucopolysaccharidoses, which is caused by mutation of the gene encoding iduronate 2-sulfatase (IDS). The loss of function of IDS leads to the accumulation of heparan sulfate and dermatan sulfate of glycosaminoglycans throughout the body, resulting in skeletal deformities, mental retardation, rigid joints, and thick skin. Recently, enzyme replacement therapy has become a common strategy for treating this condition. However, its effectiveness on the central nervous system (CNS) is limited because intravenously administered recombinant IDS (rIDS) cannot pass through the blood brain barrier. Therefore, several methods for delivering rIDS to the CNS, using anti-human transferrin receptor antibody and adeno-associated virus 9, have been explored. To investigate additional approaches for treatment, more cognition about the intracellular dynamics of mutant IDS is essential. We have already found that mutant IDS accumulated in the endoplasmic reticulum (ER) and was degraded by ER-associated degradation (ERAD). Although the dynamics of degradation of mutant IDS was revealed, the molecular mechanism related to the folding of mutant IDS in the ER remained unclear. In this research, we confirmed that mutant IDS retained in the ER would be folded by binding with calnexin (CNX). Thus, knockdown of CNX reduced the translocation of mutant IDS from ER to lysosome and its enzyme activity, indicating that the correct folding of this protein via interaction with CNX ensures its functional activity. These findings reveal the possibility that modifying the interaction of mutant IDS and CNX could contribute to alternative therapeutic strategies for MPS II.

Keywords: Calnexin cycle; ER-associated degradation; Iduronate 2-sulfatase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaloids / pharmacology
  • Calnexin / metabolism*
  • Endoplasmic Reticulum / drug effects
  • Endoplasmic Reticulum / metabolism
  • Endoplasmic Reticulum-Associated Degradation
  • Glycoproteins / chemistry
  • Glycoproteins / genetics*
  • Glycoproteins / metabolism
  • HeLa Cells
  • Humans
  • Lysosomes / metabolism
  • Mucopolysaccharidosis II / genetics
  • Mutation
  • Protein Folding
  • Ubiquitin-Protein Ligases / genetics
  • Ubiquitin-Protein Ligases / metabolism

Substances

  • Alkaloids
  • CANX protein, human
  • Glycoproteins
  • IDS protein, human
  • kifunensine
  • Calnexin
  • SYVN1 protein, human
  • Ubiquitin-Protein Ligases