Abstract
Prostate cancer (PCa) is the second cause of death due to malignancy among men, and metastasis is the leading cause of mortality in patients with PCa. MicroRNAs (miRNAs) play important regulatory roles in tumor development and metastasis. Here, we identified 13 miRNAs related to PCa metastasis by bioinformatics analysis. Moreover, we found that miR-671-5p was increased in metastatic PCa tissues, and its high expression indicated poor prognosis of PCa. MiR-671-5p could facilitate PCa cells proliferation, migration, and invasion in vitro and vivo. We confirmed that miR-671-5p directly bound to the 3' untranslated regions of NFIA mRNA, and NFIA directly bound to the CRYAB promoter. High expression of NFIA and CRYAB negatively correlated with the advanced clinicopathological characteristics and metastasis status of PCa patients. Our study demonstrated that miR-671-5p promoted PCa development and metastasis by suppressing NFIA/ CRYAB axis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Apoptosis
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Biomarkers, Tumor / genetics
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Biomarkers, Tumor / metabolism*
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Cell Proliferation
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Gene Expression Regulation, Neoplastic*
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Humans
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Lung Neoplasms / genetics
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Lung Neoplasms / metabolism
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Lung Neoplasms / secondary*
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Male
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Mice
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Mice, Inbred BALB C
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Mice, Nude
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MicroRNAs / genetics*
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NFI Transcription Factors / antagonists & inhibitors*
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NFI Transcription Factors / genetics
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NFI Transcription Factors / metabolism
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Prognosis
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Prostatic Neoplasms / genetics
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Prostatic Neoplasms / metabolism
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Prostatic Neoplasms / pathology*
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Survival Rate
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Tumor Cells, Cultured
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Xenograft Model Antitumor Assays
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alpha-Crystallin B Chain / antagonists & inhibitors*
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alpha-Crystallin B Chain / genetics
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alpha-Crystallin B Chain / metabolism
Substances
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Biomarkers, Tumor
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CRYAB protein, human
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MIRN671 microRNA, human
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MicroRNAs
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NFI Transcription Factors
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NFIA protein, human
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alpha-Crystallin B Chain