Abstract
Optical tweezers enable the isolation and mechanical manipulation of individual nucleoprotein complexes. Here, we describe how to use this technique to interrogate the mechanical properties of individual protein-DNA complexes and extract information about their overall structural organization.
Keywords:
Optical tweezers; Protein-DNA organization; SSB; Single-molecule manipulation; Single-stranded DNA.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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DNA Replication
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DNA, Single-Stranded / chemistry
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DNA, Single-Stranded / metabolism*
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / metabolism*
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Humans
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Mitochondrial Proteins / chemistry
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Mitochondrial Proteins / metabolism*
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Molecular Conformation
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Nucleoproteins / chemistry*
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Optical Tweezers
Substances
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DNA, Single-Stranded
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DNA-Binding Proteins
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Mitochondrial Proteins
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Nucleoproteins
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NABP2 protein, human