Association of MIR17HG and MIR155HG gene variants with steroid-induced osteonecrosis of the femoral head in the population of northern China

J Orthop Surg Res. 2021 Nov 15;16(1):673. doi: 10.1186/s13018-021-02669-y.

Abstract

Introduction: Steroid-induced osteonecrosis of the femoral head (ONFH) is a disease of the bone. Metabolism and genetic factors are generally considered to play an important role. The purpose of this study was to investigate the relationship between single-nucleotide polymorphisms (SNPs) in MIR17HG and MIR155HG and the risk of steroid-induced ONFH in the population of northern China.

Methods: A total of 199 steroid-induced ONFH patients and 506 healthy controls were recruited for the study. Four SNPs of MIR17HG and seven SNPs of MIR155HG were genotyped by Sequenom MassARRAY. ORs and 95% CIs were used to evaluate the relationship between these SNPs and steroid-induced ONFH.

Results: In the codominant model, patients with the MIR17HG SNPs (rs7318578) AA genotype had an increased risk of steroid-induced ONFH (OR = 1.79, p = 0.039); in the recessive model, patients with the MIR17HG SNP (rs7318578) AA genotype had an increased risk of steroid-induced ONFH (OR = 1.78, p = 0.032). Stratified analysis showed that a MIR17HG SNP (rs7318578) and the MIR155HG SNPs (rs77218221, rs11911469, rs34904192 and rs4143370) were closely related to different unornamented phenotypes of steroid-induced ONFH. Analysis of the clinical indicators revealed significant differences in high-density lipoprotein (HDL-C) levels between the ONFH group and the control group (p = 0.005). In the MIR17HG SNP (rs75267932), patients with different genotypes had different levels of triglyceride (TG). The MIR155HG SNPs (rs77699734, rs1893650, and rs34904192) showed differences in triglyceride (TG), high-density lipoprotein (HDL-C) and low-density lipoprotein (LDL-C) levels in patients with different genotypes.

Conclusion: Our results confirm that MIR17HG and MIR155HG gene mutations are associated with steroid-induced ONFH susceptibility in the population of northern China, providing new evidence for the early detection and prevention of ONFH.

Keywords: Case–control study; MIR155HG; MIR17HG; Single-nucleotide polymorphism; Steroid-induced osteonecrosis of the femoral head.

MeSH terms

  • Asian People / genetics
  • Case-Control Studies
  • China / epidemiology
  • Femur Head Necrosis* / chemically induced
  • Femur Head Necrosis* / epidemiology
  • Femur Head Necrosis* / genetics
  • Femur Head*
  • Genetic Predisposition to Disease
  • Humans
  • Lipoproteins, HDL / chemistry
  • Lipoproteins, HDL / metabolism
  • MicroRNAs
  • Polymorphism, Single Nucleotide / genetics
  • RNA, Long Noncoding
  • Steroids / adverse effects
  • Triglycerides / chemistry
  • Triglycerides / metabolism

Substances

  • Lipoproteins, HDL
  • MIR17HG, human
  • MIRN155 microRNA, human
  • MicroRNAs
  • RNA, Long Noncoding
  • Steroids
  • Triglycerides