Objective: To explore the expression of regulatory T cells (Tregs) and interleukin-35 (IL-35) in peripheral blood of patients with newly diagnosed acute myeloid leukemia (AML).
Methods: Peripheral blood samples were collected from 33 newly diagnosed AML patients and 40 healthy volunteers. The levels of innate and adaptive immune cells and Tregs were detected by flow cytometry. Plasma IL-35 level was detected by enzyme-linked immunosorbent assay (ELISA). The expression levels of P35 and EBI3 mRNA were detected by RT-PCR. The correlation between the levels of Tregs and IL-35 was analyzed.
Results: The proportion of CD4+T cells, CD8+T cells, B cells and NK cells in peripheral blood of newly diagnosed AML patients were significantly lower than those of control group (P<0.05). The levels of Tregs, IL-35, and the expression of P35 mRNA and EBI3 mRNA were significantly higher than that of healthy control group (P<0.01). The Tregs level was positively correlated with plasma IL-35 level (r=0.668,P<0.01).
Conclusion: AML patients have a deficiency in immune function, which is characterized by the high levels of Tregs and IL-35 in peripheral blood, and it might be a new target for the treatment of AML.
题目: 初诊急性髓系白血病患者外周血中Tregs和IL-35表达的研究.
目的: 探讨初诊急性髓系白血病（AML）患者外周血中调节性T细胞（Regulatory T Cells, Tregs）和白介素-35（IL-35）的表达.
方法: 收集33例初诊AML患者和40例健康体检者的外周血，采用流式细胞术检测受试者外周血中固有免疫和适应性免疫细胞、Tregs的水平，酶联免疫吸附试验(ELISA)方法检测血浆IL-35水平，RT-PCR法检测P35 mRNA和EBI3 mRNA相对表达量，并分析Tregs与IL-35的相关性.
结果: 初诊AML患者外周血中CD4+T细胞、CD8+T细胞、B细胞以及NK细胞比例较对照组显著降低（P<0.05）。Tregs、IL-35水平以及P35 mRNA、EBI3 mRNA表达量较对照组显著升高（P<0.01）。AML患者外周血中Tregs与IL-35表达水平呈正相关（r=0.668，P<0.01）.
结论: AML患者存在免疫功能缺陷，Tregs和IL-35可能参与了AML患者抑制性肿瘤免疫微环境的形成，可能成为AML治疗的新靶点.
Keywords: Tregs; acute myeloid leukemia; immunosuppression; interleukin-35.