Abstract
Rex of human T-cell leukemia virus type I (HTLV-I) and Rev of human immunodeficiency virus 1 (HIV-1) are post-transcriptional regulators of viral gene expression. By means of affinity chromatography, we purified an 18-kDa cellular protein that bound to the conserved leucine-motif/activation domain of HTLV-I Rex or HIV-1 Rev. The protein that was purified through a Rev-affinity column was found to bind to Rex immunoprecipitated with anti-Rex IgG from an HTLV-I-producing cell line. We analyzed the purified approximately 18-kDa protein biochemically and identified it as prothymosin alpha. The binding activity of prothymosin alpha to Rev or Rex was completely abolished when the epsilon-amino groups of its lysine residues were chemically modified by N-succinimidyl-3-(4-hydroxy-3,5-diodo- phenyl)propionate. The functional relationship between the nuclear protein prothymosin alpha and Rex-Rev is discussed.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Binding Sites
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Carrier Proteins / genetics
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Carrier Proteins / isolation & purification
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Carrier Proteins / metabolism
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Cell Line
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Chromatography, Affinity
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Gene Products, rev / genetics
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Gene Products, rev / isolation & purification
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Gene Products, rev / metabolism*
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Gene Products, rex / genetics
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Gene Products, rex / isolation & purification
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Gene Products, rex / metabolism*
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HIV-1 / chemistry
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HIV-1 / genetics
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HIV-1 / metabolism*
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Human T-lymphotropic virus 1 / chemistry
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Human T-lymphotropic virus 1 / genetics
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Human T-lymphotropic virus 1 / metabolism*
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Humans
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Molecular Sequence Data
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Precipitin Tests
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Protein Binding
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Protein Precursors / genetics
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Protein Precursors / isolation & purification
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Protein Precursors / metabolism*
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Sequence Homology, Amino Acid
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Thymosin / analogs & derivatives*
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Thymosin / genetics
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Thymosin / isolation & purification
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Thymosin / metabolism
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rev Gene Products, Human Immunodeficiency Virus
Substances
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Carrier Proteins
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Gene Products, rev
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Gene Products, rex
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Protein Precursors
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prothymosin alpha
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rev Gene Products, Human Immunodeficiency Virus
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Thymosin