Abstract
p53 has pleiotropic functions including control of genomic plasticity and integrity. Here we report that p53 can bind to several transcription factor IIH-associated factors, including transcription-repair factors, XPD (Rad3) and XPB, as well as CSB involved in strand-specific DNA repair, via its C-terminal domain. We also found that wild-type, but not Arg273His mutant p53 inhibits XPD (Rad3) and XPB DNA helicase activities. Moreover, repair of UV-induced dimers is slower in Li-Fraumeni syndrome cells (heterozygote p53 mutant) than in normal human cells. Our findings indicate that p53 may play a direct role in modulating nucleotide excision repair pathways.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphatases / genetics
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Adenosine Triphosphatases / metabolism
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Amino Acid Sequence
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Chromosome Mapping
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Cockayne Syndrome / enzymology
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Cockayne Syndrome / genetics
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DNA Helicases / genetics
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DNA Helicases / metabolism
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DNA Repair Enzymes
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DNA Repair*
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Enzyme Activation
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Humans
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Li-Fraumeni Syndrome / enzymology
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Li-Fraumeni Syndrome / genetics
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Models, Molecular
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Molecular Sequence Data
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Nucleotides
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Poly-ADP-Ribose Binding Proteins
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Protein Structure, Secondary
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Proteins / genetics
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Proteins / metabolism
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Saccharomyces cerevisiae Proteins
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Transcription Factor TFIIH
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Transcription Factors / metabolism
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Transcription Factors / physiology*
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Transcription Factors, TFII*
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Tumor Cells, Cultured
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Tumor Suppressor Protein p53 / metabolism
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Tumor Suppressor Protein p53 / physiology*
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Xeroderma Pigmentosum / enzymology
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Xeroderma Pigmentosum / genetics
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Xeroderma Pigmentosum Group D Protein
Substances
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DNA-Binding Proteins
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Nucleotides
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Poly-ADP-Ribose Binding Proteins
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Proteins
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Transcription Factors, TFII
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Tumor Suppressor Protein p53
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XPBC-ERCC-3 protein
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Transcription Factor TFIIH
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Adenosine Triphosphatases
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Rad3 protein, S cerevisiae
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DNA Helicases
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ERCC6 protein, human
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Xeroderma Pigmentosum Group D Protein
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ERCC2 protein, human
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DNA Repair Enzymes