Abstract
Autoimmune myocarditis is considered to play a major role in the pathogenesis of dilated cardiomyopathy. A new autoimmune myocarditis model was attained by repeated immunization using murine cardiac C-protein with the immunological adjuvant, Klebsiella pneumoniae O3 lipopolysaccharide. For further analysis of a pathological epitope, the cDNA encoding C-protein was isolated; a fusion protein encoded by part of this cDNA induced myocarditis in SMA mice as well as in three other strains: DBA/1J (H-2q), O20/A (H-2pz1), and SJL (H-2s). The nucleotide sequence and its deduced amino acid analysis revealed that this protein had immunoglobulin-like and fibronectin-like repeats. This study provides a new animal model of autoimmune myocarditis which may shed light on the pathogenesis of dilated cardiomyopathy.
MeSH terms
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Adult
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Aged
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Amino Acid Sequence
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Animals
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Autoantibodies / blood*
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Autoimmune Diseases / chemically induced
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Autoimmune Diseases / pathology*
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Cardiomyopathy, Dilated / blood
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Cardiomyopathy, Dilated / immunology*
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Carrier Proteins
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Cloning, Molecular
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DNA, Complementary / metabolism
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Female
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Heart / drug effects
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Humans
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Inflammation
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Klebsiella pneumoniae
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Lipopolysaccharides / administration & dosage
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Lipopolysaccharides / toxicity*
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Male
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Mice
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Mice, Inbred DBA
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Mice, Inbred Strains
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Middle Aged
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Muscle Proteins / biosynthesis
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Muscle Proteins / toxicity*
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Myocarditis / chemically induced
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Myocarditis / immunology*
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Myocarditis / pathology*
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Myocardium / pathology*
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Proteins / toxicity
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Reference Values
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Restriction Mapping
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Sequence Homology, Amino Acid
Substances
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Autoantibodies
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Carrier Proteins
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DNA, Complementary
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Lipopolysaccharides
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Muscle Proteins
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Recombinant Fusion Proteins
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Recombinant Proteins
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myosin-binding protein C