Abstract
A gene was cloned from Actinobacillus pleuropneumoniae strain 4074 by complementation of an aroD strain of Escherichia coli. The E. coli gene aroD codes for a 3-dehydroquinase enzyme of type I, active in the aromatic biosynthesis pathway. The A. pleuropneumoniae gene, termed aroQ, displays no base or amino acid sequence homology to aroD of E. coli. It is instead homologous to the QUTE and qa-2 genes, respectively of Aspergillus nidulans and Neurospora crassa. These genes code for 3-dehydroquinase enzymes of type II, involved in the catabolism of quinic acid. The 1.8 kb fragment, which includes aroQ, carries four overlapping or adjacent open reading frames: a dapD gene; aroQ; one without homology to sequences in GenBank; and one with homology to the C-terminal 40% of chIN of E. coli.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Actinobacillus pleuropneumoniae / enzymology*
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Actinobacillus pleuropneumoniae / genetics*
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Amino Acid Sequence
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Aspergillus nidulans / enzymology
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Aspergillus nidulans / genetics*
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Base Sequence
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Cloning, Molecular
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Escherichia coli
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Genes, Bacterial*
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Genes, Fungal
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Genetic Complementation Test
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Hydro-Lyases / biosynthesis
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Hydro-Lyases / genetics*
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Molecular Sequence Data
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Mycobacterium tuberculosis / enzymology
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Mycobacterium tuberculosis / genetics
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Neurospora crassa / enzymology
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Neurospora crassa / genetics*
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Open Reading Frames
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Plasmids
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Restriction Mapping
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Salmonella typhimurium / enzymology
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Salmonella typhimurium / genetics
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Sequence Homology, Amino Acid
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Shigella flexneri / enzymology
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Shigella flexneri / genetics
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Species Specificity
Substances
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Hydro-Lyases
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3-dehydroquinate dehydratase
Associated data
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GENBANK/L19895
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GENBANK/X63201