Abstract
The Dunning rat prostatic carcinoma is a model system where cell motility closely correlates with the metastatic phenotype. We have identified a novel gene, upregulated in the highly motile and metastatic Dunning cancer cell lines, that represents a new member of the thymosin-beta family, thymosin beta 15. Transfection of antisense thymosin beta 15 constructs into rat prostatic carcinoma cells demonstrates that this molecule positively regulates cell motility, a critical component of the metastatic pathway. Thymosin beta 15 levels are elevated in human prostate cancer and correlate positively with the Gleason tumor grade. Thymosin beta 15 may represent a potential new biochemical marker for human prostate cancer progression.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Actins / metabolism
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Amino Acid Sequence
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Animals
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Base Sequence
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Biomarkers, Tumor / analysis
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Carcinoma / chemistry
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Carcinoma / genetics
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Carcinoma / pathology*
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Cell Movement / physiology*
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Cloning, Molecular
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Gene Expression Regulation, Neoplastic / physiology*
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Humans
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Hyperplasia
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Male
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Molecular Sequence Data
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Neoplasm Metastasis
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Organ Specificity
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Prostate / chemistry
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Prostate / pathology
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Prostatic Neoplasms / chemistry
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Prostatic Neoplasms / genetics
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Prostatic Neoplasms / pathology*
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RNA, Antisense
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RNA, Messenger / analysis
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RNA, Neoplasm / analysis
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Rats
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Recombinant Fusion Proteins
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Thymosin / analysis
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Thymosin / genetics
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Thymosin / pharmacology
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Thymosin / physiology*
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Tumor Cells, Cultured
Substances
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Actins
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Biomarkers, Tumor
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RNA, Antisense
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RNA, Messenger
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RNA, Neoplasm
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Recombinant Fusion Proteins
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thymosin beta15, rat
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Thymosin