Abstract
Basal transcription from the human RNA polymerase III U6 promoter depends on a TATA box that recruits the TATA box-binding protein (TBP) and a proximal sequence element that recruits the small nuclear RNA (snRNA)-activating protein complex (SNAPc). TBP consists of a conserved carboxyl-terminal domain that performs all known functions of the protein and a nonconserved amino-terminal region of unknown function. Here, the amino-terminal region is shown to down-regulate binding of TBP to the U6 TATA box, mediate cooperative binding with SNAPc to the U6 promoter, and enhance U6 transcription.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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DNA Footprinting
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Down-Regulation
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Humans
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Molecular Sequence Data
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Promoter Regions, Genetic*
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Proteins / metabolism
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RNA, Small Nuclear / genetics*
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RNA-Binding Proteins / metabolism
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / metabolism
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TATA Box
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TATA-Box Binding Protein
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Transcription Factors / chemistry
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcription, Genetic*
Substances
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DNA-Binding Proteins
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Proteins
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RNA, Small Nuclear
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RNA-Binding Proteins
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Recombinant Fusion Proteins
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SNAPC1 protein, human
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SNAPC2 protein, human
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SNAPC3 protein, human
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TATA-Box Binding Protein
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Transcription Factors